Articles #
This section hosts a curated collection of written works, including research papers, white papers, and analytical documents. The articles cover topics such as New Approach Methodologies (NAM), toxicology, safety assessment, and regulatory science.
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Organization & Navigation
To maintain maximum information density and transparency, this repository is organized directly by issuing source (peer-reviewed scientific journals, government bodies, and intergovernmental regulatory agencies). This approach provides the orign for every document and aligns directly with primary scientific literature.
Rather than utilizing complex, nested page routing or heavy visual filtering systems, this repository implements a lightweight, text-based indexing system using hashtags. This keeps the repository entirely flat, fast-loading, and immediately searchable.
To find specific content across the entire page instantly, simply use your browser’s native search function (Ctrl + F or Cmd + F) and enter any of the indexed hashtags listed below.
Index of Hashtags As the repository expands, new operational tags will be appended to this index in alphabetical order.
- #biometrics - Computational models, bioinformatics, multi-omic statistical pipelines, and quantitative analyses used to map biological data.
- #bioprinting - Advanced 3D fabrication technologies, tissue engineering scaffolds, and bio-manufacturing processes.
- #chemical-testing - Studies detailing specific experimental assays, validation data, and methodology protocols for chemical toxicity screening.
- #data-federation - Projects focused on the integration, harmonization, and interoperability of fragmented data repositories.
- #ethics - Philosophical, legal, and moral arguments surrounding animal testing and the transition to human-relevant science.
- #future-food - Specifically tracking cultured tissue, alternative proteins, and cellular agricultural scaffolds
- #guidelines - Official protocols, procedural standards, and compliance frameworks for non-animal research.
- #invitro - Studies and documentation focusing on cell cultures, tissues, organs-on-a-chip, and other isolated biological systems.
- #neuroscience - For research and assays focused on neural tissue engineering, brain organoids, and neurotoxicity testing.
- #organ-on-a-chip - For microphysiological systems (MPS) that recapitulate organ-level function on microfluidic platforms.
- #pharmacology - For studies involving drug mechanism, transport kinetics, and therapeutic efficacy.
- #regulatory - Documents covering the legal implementation, acceptance, and standardization of NAMs by government oversight bodies.
- #research - Peer-reviewed scientific papers detailing primary experimental data, methodologies, and technical validation.
- #safety-testing - Practical applications of NAMs within industrial, chemical, consumer product, and environmental risk assessment frameworks.
- #translation - Analyses focusing on human relevance, clinical translatability, and the predictive failure rates of legacy animal models.
- #wearables - For skin and eye diagnostic tracking devices
- #whitepaper - Strategic roadmaps, institutional reports, and comprehensive policy overviews issued by agencies or organizations.
ATLA - Alternatives to Laboratory Animals #
An international, peer-reviewed journal publishing articles on the development, validation, and implementation of methods that replace, reduce, or refine the use of live animals in biomedical research and toxicity testing. [Link]
A History of Regulatory Animal Testing: What Can We Learn?
#research #regulatory #translation
Doortje Swaters, Anne van Veen, Wim van Meurs, et al.
An archival examination of how animal testing became institutionalized in regulatory policy, offering insights on how to dismantle systemic reliance on legacy methods.
A Review of In Silico Tools as Alternatives to Animal Testing: Principles, Resources and Applications
#research #safety-testing
Judith C. Madden, Steven J. Enoch, Alicia Paini, et al.
A baseline reference guide outlining the core computational tools, chemical safety databases, and predictive algorithms available to completely replace live animal screenings.
Barriers to the Use of Recombinant Bacterial Endotoxins Test Methods in Parenteral Drug, Vaccine and Device Safety Testing
#research #safety-testing #regulatory
Elizabeth Baker, Jessica Ponder, Johannes Oberdorfer, et al.
Identifies the commercial, bureaucratic, and international regulatory bottlenecks slowing down the adoption of non-animal recombinant testing methods for vaccines and medical devices.
Data-driven science, ethics and risk: Building knowledge of key ethics–risk intersections relevant to public sector decision-making
#research #ethics #regulatory
Yadvinder Bhuller, Marc Avey, P. Charukeshi Chandrasekera, et al.
An analysis of public sector decision-making at the intersection of data science, ethical frameworks, and risk assessment regarding safety policies.
It’s Time to Review the Three Rs, to Make them More Fit for Purpose in the 21st Century
#research #ethics #translation
Jarrod Bailey
A critical assessment of the traditional 3Rs framework, arguing for an evolutionary shift away from mere refinement or reduction toward absolute human-relevant replacement methodologies.
Poor Translatability of Biomedical Research Using Animals - A Narrative Review
#research #translation #ethics
Lindsay J. Marshall, Jarrod Bailey, Manuela Cassotta, et al.
A detailed review documenting the widespread failure of animal models to accurately predict human clinical outcomes in biomedical research.
Replacing immunisation-dependent antibody reagent production: Challenges under the UK Replacing Animals in Science 2025 strategy
#research #regulatory #guidelines
Alison C. Gray, Kevin C. Gough
Examines the strategic hurdles and technical challenges in transitioning away from animal-immunization methods for generating antibody reagents within national scientific frameworks.
ALTEX #
Alternatives to Animal Experimentation An open-access quarterly journal publishing peer-reviewed scientific papers on the development and validation of alternatives to animal testing, with a strong focus on ethics and regulatory policy. [Link]
Advancing skin sensitization potency categorization using U-SENS™ in OECD TG 497
#research #safety-testing #regulatory
Nathalie Alépée, Fleur Tourneix, Laurent Nardelli, et al.
A scientific paper demonstrating the optimization of skin sensitization potency evaluations using the U-SENS assay within existing OECD testing guidelines.
Bridging the gap in chemical risk assessment: Leveraging metabolite similarity for enhanced read-across applications
#research #safety-testing
Jenny Irwan, George E. N. Kass, Rupert Kellner, et al.
Details how computational assessments can leverage metabolite structural similarities to strengthen non-animal read-across predictions in chemical risk management.
Chemical fate in vitro: A physiological biokinetic (PBK) model for cell-based assays
#research #safety-testing
Daniela Brenner, Kévin Bernal, Eliška Sychrová, et al.
Presents a physiological biokinetic model designed to calculate chemical concentrations and distribution kinetics directly within in vitro cell-based testing environments.
IQ-MPS Affiliate perspectives on blood-brain barrier microphysiological systems: Progress and challenges ahead
#research #invitro #translation
Benoit Cox, Nikita Karra-Bhardwaj, Paresh P. Chothe, et al.
An industry and scientific review focusing on the current technological capabilities and structural bottlenecks facing human blood-brain barrier models on chips.
Lost in NAMs-lation: A review of animal-free science definitions
#research #translation #regulatory
Donna S. Macmillan, Phi Holcomb, Kristie M. Sullivan
An analysis of the competing and overlapping definitions used for non-animal and alternative methods across international scientific and regulatory communities.
Pyrogen testing at a turning point – On occasion of the 30th anniversary of the whole blood monocyte activation test
#research #safety-testing #translation
Thomas Hartung
A historical and forward-looking review marking three decades of the non-animal whole blood monocyte activation test as a superior replacement for legacy rabbit pyrogen screenings.
The Findable, Accessible, Interoperable, Reusable (FAIR) Lite Principles to ensure utility of computational toxicology models
#research #safety-testing
Mark T. D. Cronin, Homa Basiri, Samuel J. Belfield, et al.
Establishes baseline data standards to ensure that computational toxicology and predictive in silico algorithms remain functional, accessible, and shared across scientific networks.
The Merck “3 Baskets” approach for creating roadmaps to phase out animal testing
#research #regulatory #whitepaper
Kerstin Kleinschmidt-Doerr, Frederic C. Pipp, Isabelle Colmagne-Poulard, et al.
A strategic corporate report outlining an industry framework used to categorize chemical testing practices and chart actionable company roadmaps to eliminate animal usage.
Towards NAM-based risk assessment for developmental neurotoxic effects, illustrated with chlorpyrifos
#research #safety-testing #regulatory
Anne Zwartsen, Joost Westerhout, Shensheng Zhao, et al.
A case study utilizing the organophosphate pesticide chlorpyrifos to outline a blueprint for executing developmental neurotoxicity evaluations using entirely non-animal data models.
Archives of Toxicology #
A highly respected scientific journal focusing on toxicological mechanisms, which frequently publishes seminal research on computational models, microphysiological systems, and regulatory NAM frameworks. [Link]
Applicability of next-generation risk assessment generic PBK models and their performance within the chemical domain
#research #safety-testing #regulatory
M. Spaenig, M. W. Ashraf, … S. E. Escher
Evaluates the performance and domain boundaries of generic physiologically based kinetic (PBK) models as foundational instruments for human-relevant next-generation risk assessment (NGRA).
Comparison of ex vivo placenta perfusion and in vitro BeWo b30 cell models for assessing transfer of developmental and reproductive toxic compounds
#research #invitro #safety-testing
Damian Roelofsen, Sandrine Spriggs, … Rick Greupink
Compares performance benchmarks between ex vivo tissue models and human BeWo cell lines to validate robust non-animal methods for assessing reproductive toxicity.
Extrapolation of in vitro effect concentrations to in vivo bioavailable concentrations using PBK modelling in humans for two classes of persistent and mobile compounds: triazoles and triazines
#research #safety-testing
Abishek Laxmanan Ravi Shankar, Jenny Irwan, … Sylvia E. Escher
Demonstrates the deployment of in vitro ADME parameters combined with computational human PBK models to replace legacy animal models in calculating environmental compound safety barriers.
From animal models to NAMs: a paradigm shift in developmental immunotoxicity testing
#research #translation #regulatory
Martina Iulini, Véronique Bruijn, … Emanuela Corsini
Traces the systemic evolution of developmental immunotoxicity (DIT) testing, outlining a framework to transition entirely from obsolete animal studies to modern, human-centric NAM infrastructure.
High-throughput PBK modelling for dermal exposure: a pragmatic approach to predict systemic pharmacokinetics
#research #safety-testing
Zeynep Edizcan, Stephan Schaller, … René Geci
Introduces a high-throughput computational framework designed to mechanically isolate and project human dermal drug absorption and systemic kinetics without biological testing.
Integration of in vitro and in silico approaches enables prediction of drug-induced liver injury
#research #safety-testing
René Geci, Ahenk Zeynep Sayin, … Lars Kuepfer
Combines structural cell-based assays with mathematical simulations to solve a primary challenge in drug development: predicting drug-induced liver injury (DILI) before human clinical phase tracking.
Prediction of human exposure and first-pass metabolism of the fungicide trifloxystrobin using an in vitro intestinal pancreatin and Caco-2/HT29-MTX model
#research #invitro #safety-testing
Eileen Hallscheidt, Kathrin Bothe, … Marc Lamshoeft
Deploys co-cultured human intestinal cellular environments to construct predictive safety assessments for human metabolic handling of agrochemicals, bypassing classic mammalian modeling.
The use of new approach methodologies (high-throughput transcriptomics) to study nanoagrochemicals: mechanisms of toxicity of a commercial copper copper oxychloride to soil model invertebrates (Enchytraeus crypticus)
#research #safety-testing
Susana I. L. Gomes, Janeck J. Scott-Fordsmand, Mónica J. B. Amorim
Examines the impact of next-generation nanoagrochemical materials on ecological health systems using high-throughput transcriptomic tracking pipelines rather than organism-level endpoints.
The way forward for assessing the human health safety of cosmetics in the EU: Proceedings of Workshop 2
#research #regulatory #whitepaper
Vera Rogiers, Emilio Benfenati, … Aglaia Koutsodimou
A comprehensive technical summary outlining the legal pathways, validation metrics, and validation bottlenecks to maintain a non-animal cosmetic testing regimen under current EU regulations.
Biomaterials #
An international journal covering the science and clinical application of biomaterials, showcasing advanced microphysiological systems, 3D bioprinting, and stem cell technologies engineered to model human tissue physiology. [Link]
3D biomaterial matrix to support long term, full thickness, immuno-competent human skin equivalents with nervous system components
#research #invitro #bioprinting
Sarah E. Lightfoot Vidal, Kasey A. Tamamoto, Hanh Nguyen, et al.
Presents a complex 3D human skin model engineered to maintain full thickness and neurological integration over extended experimental durations without live animal hosting.
3D culture models for studying branching morphogenesis in the mammary gland and mammalian lung
#research #invitro
Bryan A. Nerger, Celeste M. Nelson
Outlines advanced 3D tissue culture templates designed to dynamically isolate and study organ development mechanics in lung and mammary tissue architectures.
3-D microwell culture of human embryonic stem cells
#research #invitro
Jeffrey C. Mohr, Juan J. de Pablo, Sean P. Palecek
A foundational protocol utilizing engineered 3D microwell substrates to enable scalable growth, morphology tracking, and uniform differentiation of human pluripotent cell lines.
3-D physiomimetic extracellular matrix hydrogels provide a supportive microenvironment for rodent and human islet culture
#research #invitro
K. Jiang, D. Chaimov, S.N. Patel, et al.
Details a bioengineered 3D hydrogel environment that mimics native tissue properties to preserve the viability and metabolic secretion profiles of human pancreatic cells in vitro.
3D printable plant protein-enriched scaffolds for cultivated meat development
#research #bioprinting #future-food
Iris Ianovici, Yedidya Zagury, Idan Redenski, et al.
Evaluates the material tuning and fabrication properties of entirely plant-based bioinks utilized to 3D print complex structural scaffolds for non-animal muscle tissue engineering.
3D printed MCT oleogel as a co-delivery carrier for curcumin and resveratrol
#research #bioprinting #future-food
M. Kavimughil, M. Maria Leena, J.A. Moses, et al.
Investigates the engineering limits and molecular protection capabilities of 3D-printed fat-mimetic oleogels designed to stabilize sensitive bioactive components in synthetic foods.
3D printed PEDOT:PSS-based conducting and patternable eutectogel electrodes for machine learning on textiles
#research #wearables
Ruben Ruiz-Mateos Serrano, Ana Aguzin, Eleni Mitoudi-Vagourdi, et al.
Outlines the synthesis of patternable, conductive eutectogels for textile integration, providing the sensor foundation needed to connect wearable electronic tracking platforms with machine learning.
A novel red-emitting aggregation-induced emission probe for determination of β-glucosidase activity
#research #future-food
Bicheng Yao, Jiamin Zhao, Siyang Ding, et al.
Introduces a responsive fluorescent probe that utilizes aggregation-induced emission to execute high-contrast enzyme tracking inside cellular food-production lines.
A strategy for screening novel umami dipeptides based on common feature pharmacophore and molecular docking
#research #future-food
Yongzhao Xiong, Xinchang Gao, Daodong Pan, et al.
Uses computational molecular docking and virtual pharmacophore screening to identify novel flavor peptides, bypassing traditional animal-derived chemical characterization.
A three-dimensional (3D) organotypic microfluidic model for glioma stem cells – Vascular interactions
#research #invitro
Danh Truong, Roberto Fiorelli, Eric S. Barrientos, et al.
Presents a microfluidic tumor-on-a-chip platform designed to model the exact physical and molecular interactions between human brain tumor stem cells and vascular arrays.
Advances in ex vivo models and lab-on-a-chip devices for neural tissue engineering
#research #invitro #translation
Sahba Mobini, Young Hye Song, Michaela W. McCrary, et al.
A comprehensive review tracing microphysiological systems, microfluidic platforms, and neural-chip platforms designed to replace legacy animal models in neurological drug discovery.
Advances in wearable electronics for monitoring human organs: Bridging external and internal health assessments
#research #wearables #translation
Vo Thi Nhat Linh, Seunghun Han, Eunhye Koh, et al.
Reviews the capacity of advanced skin and surface bioelectronics to extract deep internal organ diagnostics, providing a non-invasive translation blueprint for human health logging.
Application of a Mytilus edulis-derived promoting calcium absorption peptide in calcium phosphate cements for bone
#research #future-food
Zhe Xu, Zhixuan Zhu, Hui Chen, et al.
Details the extraction and synthetic structural deployment of an absorption peptide to optimize mineral matrix consolidation, with cross-industry relevance in food structures and bone engineering.
Advances in wearable electronics for monitoring human organs: Bridging external and internal health assessments
#research #wearables #translation
Vo Thi Nhat Linh, Seunghun Han, Eunhye Koh, et al.
Reviews the capacity of advanced skin and surface bioelectronics to extract deep internal organ diagnostics, providing a non-invasive translation blueprint for human health logging.
Biomaterials for reliable wearable health monitoring: Applications in skin and eye integration
#research #wearables
Seokkyoon Hong, Tianhao Yu, Ziheng Wang, et al.
Examines the engineering challenges of integrating flexible electronic sensors onto soft human tissues (skin and cornea) to secure continuous, long-term diagnostic tracking.
Biomimetic cardiovascular platforms for in vitro disease modeling and therapeutic validation
#research #invitro #translation
Roberto Portillo-Lara, Andrew R. Spencer, Brian W. Walker, et al.
A comprehensive analysis detailing how human heart tissues are reconstructed in vitro on hydrogel chips to create high-throughput drug screening environments that bypass animal models.
Biomimetic ion nanochannels for sensing umami substances
#research #future-food
Mingyang Li, Ninglong Zhang, Zhiyong Cui, et al.
Details the engineering of a synthetic nanochannel platform modeled directly after human taste receptors to detect and profile umami compounds electronically.
Brief exposure to directionally-specific pulsed electromagnetic fields stimulates extracellular vesicle release and is antagonized by streptomycin: A potential regenerative medicine and food industry paradigm
#research #future-food
Craig Jun Kit Wong, Yee Kit Tai, Jasmine Lye Yee Yap, et al.
Outlines an electromagnetic mechanism used to non-invasively amplify cellular factor signaling, offering a novel biomanufacturing paradigm to accelerate cultivated meat production.
Cardiovascular disease models: A game changing paradigm in drug discovery and screening
#research #invitro #translation
Houman Savoji, Mohammad Hossein Mohammadi, Naimeh Rafatian, et al.
A major review of human heart-on-a-chip technologies, microvascular loops, and tissue lattices deployed to displace historical animal-testing models in pharmaceutical screening.
Chitosan and HPMCAS double-coating as protective systems for alginate microparticles loaded with Ctx(Ile21)-Ha antimicrobial peptide to prevent intestinal infections
#research #future-food
Cesar Augusto Roque-Borda, Mauro de Mesquita Souza Saraiva, et al.
Engineers a biocompatible polymer shell delivery architecture designed to shield therapeutic antimicrobial peptides from stomach breakdown during transit.
Co-culture of human embryonic stem cells with murine embryonic fibroblasts on microwell-patterned substrates
#research #invitro
Ali Khademhosseini, Lino Ferreira, James Blumling, et al.
A foundational bioengineering paper introducing a lithographic microwell patterning technique to physically isolate and standardize stem cell growth niches in vitro.
Core-shell starch as a platform for reducing starch digestion and saturated fat intake
#research #future-food
Xiaoyang Li, Bing Hu, Ruixiang Ma, et al.
Engineers a core-shell biopolymer matrix that alters enzyme digestion dynamics, creating a scalable physical platform to manufacture healthier synthetic food products.
Cyclic flexure and laminar flow synergistically accelerate mesenchymal stem cell-mediated engineered tissue formation: Implications for engineered heart valve tissues
#research #invitro
George C. Engelmayr, Virna L. Sales, John E. Mayer, et al.
Demonstrates how dynamic mechanical stress and fluid flow inside custom bioreactors accelerate the functional maturation of non-animal engineered heart tissue.
Development of a human skeletal micro muscle platform with pacing capabilities
#research #invitro
Richard J. Mills, Benjamin L. Parker, Pauline Monnot, et al.
Engineers a human skeletal muscle microfluidic matrix featuring active electrical pacing networks to evaluate cell physiological function directly in vitro.
Dissection and enhancement of prebiotic properties of yeast cell wall oligosaccharides through metabolic engineering
#research #future-food
Suryang Kwak, Scott J. Robinson, Jae Won Lee, et al.
Applies metabolic pathway engineering to optimize yeast structures, enhancing their natural functional properties for integration into sustainable cellular agricultural pipelines.
Dynamic monitoring of a 3D-printed airway tissue model using an organic electrochemical transistor
#research #wearables #invitro
Seungjin Chai, Yunji Lee, Róisín M. Owens, et al.
Integrates organic electrochemical transistors directly with 3D-printed human airway tissue models to monitor physiological health transformations in real time without destroying the tissue block.
Effect of 3D scaffold and dynamic culture condition on the global gene expression profile of mouse embryonic stem cells
#research #invitro
Hui Liu, Jian Lin, Krishnendu Roy
A comprehensive transcriptomic analysis mapping how 3D physical matrices and fluid perfusion alter global gene expression profiles during embryonic cell lineage selection.
Effects of orthopaedic wear particles on osteoprogenitor cells
#research #translation
Stuart B. Goodman, Ting Ma, Richard Chiu, et al.
Examines the specific cellular mechanisms of implant degradation and particle-induced toxicity using localized, non-animal cell models to isolate pathogenic pathways.
Electrical stimulation increases hypertrophy and metabolic flux in tissue-engineered human skeletal muscle
#research #invitro
Alastair Khodabukus, Lauran Madden, Neel K. Prabhu, et al.
Demonstrates how chronic electrical stimulation promotes metabolic flux and tissue hypertrophy inside bioengineered human skeletal muscle fibers in vitro.
Emulsion-templated microparticles with tunable stiffness and topology: Applications as edible microcarriers for cultured meat
#research #future-food
Sam C.P. Norris, N. Stephanie Kawecki, Ashton R. Davis, et al.
Engineers tunable biopolymer microparticles to act as edible microcarriers, providing a scalable material template to multiply animal-free muscle tissues.
Epidermal secretion-purified biosensing patch with hydrogel sebum filtering membrane and unidirectional flow microfluidic channels
#research #wearables
Yuqi Wang, Ziyu Zhang, Yuqing Shi, et al.
Designs a smart skin patch featuring microfluidic channels and a selective filtering hydrogel to continuously extract and purify skin secretions for accurate health tracking.
Engineered meatballs via scalable skeletal muscle cell expansion and modular micro-tissue assembly using porous gelatin micro-carriers
#research #future-food
Ye Liu, Rui Wang, Shijie Ding, et al.
Outlines a scalable manufacturing process that utilizes porous gelatin microcarriers to expand muscle cells and assemble them into thick, non-animal meat structures.
Engineered microenvironments and microdevices for modeling the pathophysiology of type 1 diabetes
#research #invitro #translation
Matthew W. Becker, Jennifer A. Simonovich, Edward A. Phelps
Reviews the biofabrication protocols and microfluidic microdevices deployed to reconstruct pancreatic tissue microenvironments, bypassing animal models for diabetes tracking.
Evaluation of immobilized microspheres of Clonostachys rosea on Botrytis cinerea and tomato seedlings
#research #future-food
Jiayin Liu, Zhengyuan Han, Lidong An, et al.
Evaluates a protective biopolymer microsphere matrix deployed to encapsulate biological protection agents, securing agricultural crop security without synthetic chemical exposure.
From lab to wearables: Innovations in multifunctional hydrogel chemistry for next-generation bioelectronic devices
#research #wearables
Hin Kiu Lee, Ye Ji Yang, Gyan Raj Koirala, et al.
Traces the advanced chemistry used to formulate multifunctional hydrogel interfaces, serving as the essential wet link between biology and skin-mounted hardware.
Future foods: Design, fabrication and production through microfluidics
#research #future-food
Xiufeng Li, Baihao You, Ho Cheung Shum, et al.
Reviews how advanced microfluidic droplet networks are used to design, manipulate, and manufacture nutrient-dense synthetic proteins and alternative food shapes.
Gelatin MAGIC powder as nutrient-delivering 3D spacer for growing cell sheets into cost-effective cultured meat
#research #future-food
Sohyeon Park, Sungwon Jung, Moonhyun Choi, et al.
Introduces an innovative nutrient-releasing gelatin matrix that operates as a 3D spacer, enabling researchers to stack individual cell sheets into thick, structured non-animal steaks economically.
Gellan gum-gelatin scaffolds with Ca2+ crosslinking for constructing a structured cell cultured meat model
#research #future-food
Yan Chen, Linzi Li, Lin Chen, et al.
Details a calcium-crosslinked gellan gum matrix designed to support the alignment and long-term cultivation of primary muscle cell layers into structured meat models.
Harvest of quality-controlled bovine myogenic cells and biomimetic bovine muscle tissue engineering for sustainable meat production
#research #future-food
Hironobu Takahashi, Azumi Yoshida, Botao Gao, et al.
Develops an industrial protocol to harvest and verify pure bovine cell sheets, providing a reproducible bioengineering blueprint for animal-free meat production lines.
High throughput scaffold-based 3D micro-tumor array for efficient drug screening and chemosensitivity testing
#research #invitro
Xiaojun Yan, Lyu Zhou, Zhaozhao Wu, et al.
Outlines a dense 3D micro-scaffold array that automates human micro-tumor cultivation, facilitating large-scale non-animal drug screening.
High-throughput micro-tumor arrays on polymer fibers for automated drug testing
#research #invitro
Xiaojun Yan, Lyu Zhou, Zhaozhao Wu, et al.
Outlines a dense 3D micro-scaffold array that automates human micro-tumor cultivation, facilitating large-scale non-animal drug screening.
Homemade bread: Repurposing an ancient technology for in vitro tissue engineering
#research #invitro
Jessica T. Holmes, Ziba Jaberansari, William Collins, et al.
Repurposes the porous structure of baked bread as an accessible, highly porous non-animal scaffold to guide 3D cell infiltration and tissue architecture formatting.
Identification and structural analysis of novel malathion-specific DNA aptameric sensors designed for food testing
#research #future-food
Ulhas Sopanrao Kadam, Kien Hong Trinh, Vikas Kumar, et al.
Isolates novel synthetic DNA aptamer sequences tailored to detect organophosphate pesticide contamination instantly in agricultural products.
In vitro and ex vivo systems at the forefront of infection modeling and drug discovery
#research #invitro #translation
Di Shi, Gujie Mi, Mian Wang, Thomas J. Webster
A technical evaluation detailing how biomimetic chip arrays capture human pathogen interactions, rendering historical mammalian infection modeling obsolete.
In-silico investigation of umami peptides with receptor T1R1/T1R3 for the discovering potential targets: A combined modeling approach
#research #future-food
Wenli Wang, Zhiyong Cui, Menghua Ning, et al.
Combines computational taste-receptor mapping and docking algorithms to isolate novel umami peptides, bypassing legacy organism sensory panels.
Intestinal-targeted nanotubes-in-microgels composite carriers for capsaicin delivery and their effect for alleviation of Salmonella induced enteritis
#research #future-food
Yu Yuan, Ying Liu, Yang He, et al.
Deploys a specialized nanotube-in-microgel composite carrier to deliver precise capsaicin doses directly to the intestine, offering a non-chemical methodology to manage enteritis.
Intrafibrillar, bone-mimetic collagen mineralization regulates breast cancer cell adhesion and migration
#research #invitro
Siyoung Choi, Jens Friedrichs, Young Hye Song, et al.
Uses a mineralized 3D collagen platform to discover how bone structural properties influence human breast cancer cell migration, bypassing traditional mouse bone matrix grafts.
Large-scale cultured meat production: Trends, challenges and promising biomanufacturing technologies
#research #future-food
Lu Chen, Donovan Guttieres, Andrea Koenigsberg, et al.
Maps the global engineering bottlenecks and bioreactor design upgrades necessary to scale animal-free muscle tissue manufacturing to an industrial capacity.
Mechanism differences between reductive and oxidative dough rheology improvers in the formation of 1D and 3D gluten network
#research #future-food
Jihui Gao, Yizhan Guo, Rongrong Yan, et al.
Analyzes the molecular disulfide binding mechanics of plant gluten networks, establishing material parameters to engineer synthetic textures in plant-based proteins.
Microfluidic devices for disease modeling in muscle tissue
#research #invitro #translation
Mollie M. Smoak, Hannah A. Pearce, Antonios G. Mikos
Evaluates the design of microfluidic devices used to cultivate healthy and diseased human muscle fibers on chips for non-animal therapeutic evaluation.
Multifunctional nanomaterials for smart wearable diabetic healthcare devices
#research #wearables
Tae Yeon Kim, Ranjit De, Inhoo Choi, et al.
Details the incorporation of advanced nanomaterials into non-invasive wearable devices to automate continuous blood monitoring and delivery management.
Multiscale bioprinting of vascularized models
#research #invitro #bioprinting
Amir K. Miri, Akbar Khalilpour, Berivan Cecen, et al.
Details advanced multi-nozzle 3D bioprinting techniques designed to construct complex fluid networks inside engineered human organ models.
Natural biomaterials for sustainable flexible neuromorphic devices
#research #wearables
Yanfei Zhao, Seungbeom Lee, Tingyu Long, et al.
Repurposes natural, plant-derived biopolymers to construct flexible electronic neuromorphic circuits, advancing sustainable hardware for skin-mounted diagnostic networks.
Natural biopolymer masks the bitterness of potassium chloride to achieve a highly efficient salt reduction for future foods
#research #future-food
Wei Lu, Zining Hu, Xuelian Zhou, et al.
Uses plant-derived biopolymer coatings to mask mineral taste properties, solving a common formulation challenge in clean-label alternative foods.
New vegetable-waste biomaterials by Lupin albus L. as cellular scaffolds for applications in biomedicine and food
#research #future-food #invitro
Silvia Buonvino, Matteo Ciocci, Francesca Nanni, et al.
Extracts structural cellulose matrices from agricultural lupin plant waste to fabricate sustainable, edible 3D scaffolds for cellular agriculture and tissue models.
Optimization of fibrin scaffolds for differentiation of murine embryonic stem cells into neural lineage cells
#research #invitro
Stephanie M. Willerth, Kelly J. Arendas, David I. Gottlieb, et al.
Optimizes the density and growth-factor loading of non-animal fibrin hydrogels to accurately drive stem cell colonies into functional neural tissues.
Perivascular signals alter global gene expression profile of glioblastoma and response to temozolomide in a gelatin hydrogel
#research #invitro
Mai T. Ngo, Brendan A.C. Harley
Uses a non-animal gelatin hydrogel framework to demonstrate how vascular signals modify cancer genes and therapeutic resistance, providing an alternative to mouse brain tumor grafts.
Perspectives on scaling production of adipose tissue for food applications
#research #future-food
John S.K. Yuen Jr, Andrew J. Stout, N. Stephanie Kawecki, et al.
Examines the specific cultivation conditions, raw material constraints, and fat cell scaling protocols needed to manufacture authentic animal-free lipids for clean meat.
Production of cultured meat from pig muscle stem cells
#research #future-food
Haozhe Zhu, Zhongyuan Wu, Xi Ding, et al.
Establishes a defined protocol to isolate, proliferate, and structurally organize porcine satellite muscle lines into authentic cultured meat without animal serum.
Production of food-grade microcarriers based on by-products from the food industry to facilitate the expansion of bovine skeletal muscle satellite cells for cultured meat production
#research #future-food
R. Christel Andreassen, Sissel Beate Rønning, et al.
Repurposes food industry protein processing side-streams into food-grade microcarriers to maximize cellular surface area expansion in industrial agriculture bioreactors.
Quercetin directed transformation of calcium carbonate into porous calcite and their application as delivery system for future foods
#research #future-food
Tian Lan, Yabo Dong, Zejian Xu, et al.
Uses the natural plant compound quercetin to crystallize porous, food-grade calcite arrays, providing a stable microscopic vehicle to encapsulate nutrients.
Smart filtering facepiece respirator with self-adaptive fit and wireless humidity monitoring
#research #wearables
Kangkyu Kwon, Yoon Jae Lee, Yeongju Jung, et al.
Integrates wireless flexible electronics and adaptive polymer components into specialized facepieces to achieve self-adjusting seals and real-time respiratory logging.
Stem cell-based tissue engineering with silk biomaterials
#research #invitro #translation
Yongzhong Wang, Hyeon-Joo Kim, Gordana Vunjak-Novakovic, David L. Kaplan
A prominent review assessing how biocompatible silk fibroin proteins are processed into porous 3D scaffolds to systematically guide stem cell colonies into complex bone and cartilage lines.
Stem cells and adipose tissue engineering
#research #invitro
Cheryl T. Gomillion, Karen J.L. Burg
A comprehensive analysis mapping the material design rules, cell seeding techniques, and monitoring criteria used to construct functional human fat tissue equivalents in vitro.
Strategies to improve meat-like properties of meat analogs meeting consumers’ expectations
#research #future-food
Yan Ping Chen, Xi Feng, Imre Blank, et al.
Reviews the macromolecular crosslinking methods and formulation advancements deployed to mimic animal muscle fiber texturing in clean-label plant protein products.
Structure remodeling of soy protein-derived amyloid fibrils mediated by epigallocatechin-3-gallate
#research #future-food
Zejian Xu, Guancheng Shan, Nairong Hao, et al.
Investigates how the green tea extract EGCG shapes plant-derived amyloid fibrils, providing a mechanical method to customize protein texturing in alternative foods.
Surface-aminated electrospun nanofibers enhance adhesion and expansion of human umbilical cord blood hematopoietic stem/progenitor cells
#research #invitro
Kian-Ngiap Chua, Chou Chai, Peng-Chou Lee, et al.
Demonstrates that surface-amination of synthetic polymer nanofibers optimizes the attachment and growth of human cord blood stem cell populations in vitro.
The effects of monocytes on tumor cell extravasation in a 3D vascularized microfluidic model
#research #invitro
A. Boussommier-Calleja, Y. Atiyas, K. Haase, et al.
Uses a vascularized 3D microfluidic chip to track exactly how circulating white blood cells influence cancer cell escape routes, completely replacing live animal vascular mapping.
The intracellular fate and transport mechanism of shape, size and rigidity varied nanocarriers for understanding their oral delivery efficiency
#research #future-food
Xin Li, Seid Mahdi Jafari, Feibai Zhou, et al.
Details how the physical architecture (geometry and stiffness) of biopolymer nanocarriers controls their absorption pathways through human intestinal walls in vitro.
The kinetic mechanism of cations induced protein nanotubes self-assembly and their application as delivery system
#research #future-food
Jipeng Zhang, Qimeng Wang, Bin Liu, et al.
Details the self-assembly mechanics of plant-derived protein nanotubes under ionic control, creating clean delivery tools for structural food additions.
Three-dimensional culture for expansion and differentiation of mouse embryonic stem cells
#research #invitro
Hui Liu, Scott F. Collins, Laura J. Suggs
Demonstrates that encapsulating stem cell colonies inside porous, un-linked 3D hydrogel beds supports uniform differentiation cascades without animal-derived serum additives.
Tissue engineered bone mimetics to study bone disorders ex vivo: Role of bioinspired materials
#research #invitro #translation
Yuru Vernon Shih, Shyni Varghese
Deploys a bioinspired, cell-laden matrix to build functional bone copies in vitro, providing a reliable alternative platform to model skeletal disease pathology without testing on live animals.
Umami polypeptide detection system targeting the human T1R1 receptor and its taste-presenting mechanism
#research #future-food
Chuanxi Zhang, Yulu Miao, Yinghui Feng, et al.
Engineers an entry screening architecture based on human taste receptor profiles to isolate and verify savory compounds for advanced non-animal food products.
Computational Toxicology #
A specialized journal dedicated to the development of computer-based data models, quantitative structure-activity relationship (QSAR) models, and mathematical simulations to replace biological animal screening. [Link]
A comparative study of biostatistical pipelines for benchmark concentration modeling of in vitro screening assays
#research #invitro #safety-testing
Kelly E. Carstens, Arif Dönmez, Ellen Fritsche
Compares statistical data analysis pipelines for modeling concentration-effect thresholds in high-throughput in vitro toxicity screening arrays.
A developmental and reproductive toxicity adverse outcome pathway network to support safety assessments
#research #safety-testing #translation
Alun Myden, Alex Cayley, Adrian Fowkes
Details the creation of an adverse outcome pathway (AOP) network designed to evaluate developmental and reproductive risks without using legacy animal assays.
A matter of trust: Learning lessons about causality will make qAOPs credible
#research #regulatory #translation
Nicoleta Spînu, Mark T. D. Cronin, Andrew P. Worth
Examines the causal validation metrics required to establish international scientific and regulatory trust in quantitative adverse outcome pathways (qAOPs).
A review of in silico toxicology approaches to support the safety assessment of cosmetics-related materials
#research #safety-testing #regulatory
Mark T. D. Cronin, Steven J. Enoch, Chihae Yang
Provides a targeted review of modern computational modeling platforms and database structures utilized to evaluate cosmetics ingredients under animal-testing bans.
A transcriptomics-driven quantitative adverse outcome pathway network model for gentamicin-induced nephrotoxicity
#research #safety-testing #translation
Filippo Di Tillio, Tianqi Zhang, Joost B. Beltman
Integrates human transcriptomic cellular data into a computational network model to quantitatively project drug-induced kidney toxicity without animal cross-validation.
An exchange standard for FAIR PBK models in chemical risk assessment – A PARC community effort
#research #safety-testing #regulatory
J. W. Kruisselbrink, J. Minnema, J. Engel
Outlines an international standardized data-exchange framework designed to make physiologically based kinetic (PBK) software models findable, accessible, interoperable, and reusable (FAIR).
Application of new approach methodologies: ICE tools to support chemical evaluations
#research #safety-testing #regulatory
Jaleh Abedini, Bethany Cook, Nicole Kleinstreuer
Demonstrates how the Integrated Chemical Environment (ICE) computational toolkit can be applied to streamline non-animal chemical safety indexing and hazard profiling.
Applying high throughput toxicokinetics (HTTK) to per- and polyfluoro alkyl substances (PFAS)
#research #safety-testing
John F. Wambaugh, Rogelio Tornero-Velez, Barbara A. Wetmore
Applies high-throughput mathematical toxicokinetic models to profile the human absorption and clearance rates of diverse PFAS chemical structural groups without mammalian exposures.
Causality and the use of adverse outcome pathways to structure expert review of carcinogenicity data
#research #regulatory
Susanne A. Stalford, Alex N. Cayley, Christopher G. Barber
Demonstrates how AOP frameworks can organize a structured weight-of-evidence analysis for chemical carcinogenicity hazard profiling under regulatory guidelines.
COSMOS next generation – A public knowledge base leveraging chemical and biological data to support the regulatory assessment of chemicals
#research #safety-testing #regulatory
C. Yang, M. T. D. Cronin, A. P. Worth
Introduces an advanced public data warehouse designed to systematically synthesize chemical structures with non-animal biological endpoints for open regulatory acceptance.
Current status and future directions for a neurotoxicity hazard assessment framework that integrates in silico approaches
#research #safety-testing #translation
Kevin M. Crofton, Arianna Bassan, Glenn J. Myatt
Identifies the technical milestones and infrastructural upgrades needed to implement an entirely computer-driven hazard identification roadmap for developmental neurotoxicity.
Development and evaluation of explainable QSAR models to predict chemical-induced respiratory irritation
#research #safety-testing
Pinyi Lu, Souvik Dey, Mohamed Diwan M. AbdulHameed
Presents highly transparent, explainable quantitative structure-activity relationship (QSAR) algorithms designed to predict chemical inhalation hazards without using acute animal lung exposures.
Development of chemical categories for per- and polyfluoroalkyl substances (PFAS) and the proof-of-concept approach to the identification of potential candidates for tiered toxicological testing and human health assessment
#research #safety-testing #regulatory
G. Patlewicz, R. S. Judson, R. S. Thomas
Groups diverse structural classes of PFAS chemicals using mathematical grouping filters to map candidate compounds for automated, animal-free toxicology screens.
Development of the toxicity values database, ToxValDB: A curated resource for experimental and derived human health-relevant toxicity data
#research #safety-testing
Jonathan T. Wall, Risa R. Sayre, Chelsea A. Weitekamp
Details the compilation and curation parameters of the EPA’s public ToxValDB registry, a foundational engine for sourcing clean toxicological benchmarks to power computational algorithms.
Federation of toxicological data resources for in silico new approach methodologies (NAMs)
#research #safety-testing #regulatory
Nicoleta Spînu, Dimitris Stripelis, Andrew P. Worth
Presents a data architecture framework designed to link and share toxicological data warehouses across international computing nodes, facilitating robust in silico validation of non-animal predictive tools.
In vitro transcriptomic points of departure derived from human whole transcriptome and reduced S1500+ gene panel are highly comparable
#research #invitro #safety-testing
James Johnson, Joseph L. Bundy, Logan J. Everett
Validates that targeted, cost-effective high-throughput gene panels yield toxicological points of departure that match full-scale transcriptomic screening, optimizing large-scale NAM implementation.
In silico analyses as a tool for regulatory assessment of protein digestibility: Where are we?
#research #regulatory
Fernando Rivero-Pino, Caroline Idowu, Hannah Lester
Reviews the modern state of computer-driven protein digestion profiling software used to support structural safety and dietary evaluations under regulatory frameworks.
O-QT assistant: a multi-agent AI system for streamlined chemical hazard assessment and read-across analysis using the OECD QSAR toolbox API
#research #safety-testing
Ivo Djidrovski, Raymond Pieters, Marc Teunis
Deploys a cooperative multi-agent AI software system connected to the OECD QSAR Toolbox API to automate chemical analog selections and read-across safety
EPA #
U.S. Environmental Protection Agency A federal agency at the absolute forefront of regulatory NAM implementation, developing explicit corporate work plans, strategies, and benchmarks to eliminate vertebrate testing for chemical and environmental risk assessments. [Link]
A cheminformatics workflow to select representative TSCA chemicals for New Approach Methodology (NAM) screening | Science Inventory | US EPA
#whitepaper #safety-testing #regulatory
U.S. Environmental Protection Agency
Establishes a transparent categorization and workflow routing model designed to select 318 representative chemical candidates from the TSCA active inventory for high-throughput aqueous biological screening.
Advancing New Approach Methodologies (NAMs) for Tobacco Harm Reduction: Synopsis from the 2021 CORESTA SSPT-NAMs Symposium
#whitepaper #translation #regulatory
U.S. Environmental Protection Agency
A cross-agency overview defining the international state-of-the-science and application thresholds for deploying alternative computational and in vitro data streams to assess novel consumer nicotine configurations without mammalian screening.
Analyzing multi-dimensional developmental neurotoxicity new approach methodologies: computational approaches to identify phenotypes | Science Inventory | US EPA
#whitepaper #safety-testing
U.S. Environmental Protection Agency
Outlines mathematical and statistical pattern-recognition algorithms configured to analyze high-content imaging data, providing a scalable pathway to flag human developmental neurotoxicity variants.
Availability of New Approach Methodologies (NAMs) in the Endocrine Disruptor Screening Program (EDSP) | Science Inventory | US EPA
#whitepaper #safety-testing #regulatory
U.S. Environmental Protection Agency
An operational webinar brief defining the validation, performance metrics, and general deployment parameters of high-throughput in vitro cellular panels within the Endocrine Disruptor Screening Program framework.
Building a compendium of expert driven read-across (EDRA) cases to investigate the utility of New Approach Methodology (NAM) data in Generalized Read-Across (GenRA) | Science Inventory | US EPA
#whitepaper #safety-testing
U.S. Environmental Protection Agency
Details how an active collection of expert-curated read-across templates can serve as an objective baseline to systematically calibrate and measure the accuracy of computational GenRA algorithms.
Chemical Landscape of New Approach Methodologies for Exposure
#whitepaper #safety-testing
U.S. Environmental Protection Agency
A comprehensive technical index tracking chemical use patterns, fate boundaries, and exposure predictions, optimizing data accessibility to contextualize automated hazard testing.
Computational approaches to evaluate in vitro New Approach Methodologies (NAMs) for Developmental Neurotoxicity (DNT) | Science Inventory | US EPA
#whitepaper #safety-testing
U.S. Environmental Protection Agency
Applies supervised machine learning architectures to microelectrode arrays and neurite outgrowth datasets, demonstrating that a refined subset of informative endpoints can successfully predict in vivo toxic thresholds with 92% specificity.
December 4, 6-8, 2018 FIFRA SAP Meeting
#whitepaper #regulatory
U.S. Environmental Protection Agency
Official advisory panel proceedings reviewing an agency-proposed NAM case study engineered to completely replace complex live vertebrate testing for respiratory point-of-contact toxicity evaluations.
Development of a Tool for Integrating Traditional and New Approach Methodologies (NAMs) for Chemical Safety Decisions | Science Inventory | US EPA
#whitepaper #safety-testing #regulatory
U.S. Environmental Protection Agency
Introduces a web-based decision application that synthesizes production volumes and predictive exposure models with non-animal quantitative points of departure (PODs) to optimize TSCA chemical prioritization timelines.
ENVR 500 Environmental Processes, Exposure, and Risk Assessment: New Approach Methodologies for Chemical Risk Assessment
#whitepaper #regulatory #guidelines
U.S. Environmental Protection Agency
A core curriculum blueprint addressing the systematic integration of predictive in silico tools and in vitro screening parameters within contemporary environmental public safety architectures.
EPA is Moving Towards the Future of Chemical Assessments with New Approach Methods
#whitepaper #safety-testing
U.S. Environmental Protection Agency
An agency research briefing highlighting computational milestones and workflow designs used to isolate toxicological thresholds while safely avoiding intact animal testing models.
EPA Releases Updated New Approach Methodologies (NAMs) Work Plan
#whitepaper #regulatory #guidelines
U.S. Environmental Protection Agency
An active policy briefing documenting the regulatory benchmarks, timeline frameworks, and scientific criteria required to establish non-animal evaluation pipelines.
FIFRA SAP Meeting on “Evaluation of a Proposed Approach to Refine the Inhalation Risk Assessment for Point of Contact Toxicity A Case Study Using a New Approach Methodology (NAM).” Request for Nominations and Notice of Public Meeting
#whitepaper #regulatory
U.S. Environmental Protection Agency
A formal public notice convening an expert review panel to evaluate mathematical approaches for point-of-contact inhalation hazard assessments, advancing the practical use of NAM data streams over live mammalian assays.
New Approach Methodologies (NAMs) and Chemical Risk Assessment | Science Inventory | US EPA
#whitepaper #safety-testing #translation
U.S. Environmental Protection Agency
A high-density master presentation tracking the integration of high-throughput in vitro arrays, genomics, and toxicokinetic parameters to construct an objective, animal-free framework for chemical risk determinations.
New Approach Methodologies (NAMs) Training Pilot Program | Science Inventory | US EPA
#whitepaper #safety-testing
U.S. Environmental Protection Agency
Official registry file for the EPA’s internal training program, establishing technical competencies and instructional guidelines for practicing scientists using computer-driven predictive testing protocols.
New Approach Methods (NAMs) Training
#whitepaper #safety-testing
U.S. Environmental Protection Agency
The central training platform hosting public training libraries, workshop archives, and dynamic instructional toolkits engineered to scale computational proficiency in alternative methodologies.
New Approach Methods Work Plan
#whitepaper #regulatory #guidelines
U.S. Environmental Protection Agency
The bedrock operational work plan mapping clear, tangible agency criteria to reduce vertebrate testing requirements and actively expand the legal deployment of alternative data models.
Putting Theory into Practice: Using In Vitro and Computational New Approach Methodologies (NAMs) in Human-relevant Risk Assessment Key Human Exposure Modelling Approaches | Science Inventory | US EPA
#whitepaper #safety-testing #translation
U.S. Environmental Protection Agency
Examines technical exposure simulations and pharmacokinetic models engineered to securely extrapolate non-animal bioactivity metrics into precise human health assessments.
Strategic Vision for Adopting New Approach Methodologies - Reduction Strategies
#whitepaper #regulatory #guidelines
U.S. Environmental Protection Agency
An official strategic roadmap detailing specific data requirement adaptations and alternative methodology tools implemented to diminish animal usage throughout corporate pesticide risk profiling.
Strategic Vision for Adopting New Approach Methodologies - Replacement Strategies
#whitepaper #regulatory #guidelines
U.S. Environmental Protection Agency
Establishes clear tactical vision parameters to systematically transition pesticide safety evaluations completely away from complex vertebrate testing models toward validated, non-animal computational networks.
Strategic Vision for Adopting New Approach Methodologies - Strategic Vision for Adopting New Approach Methodologies
#whitepaper #regulatory #guidelines
U.S. Environmental Protection Agency
The structural cornerstone policy statement organizing the EPA Office of Pesticide Programs’ systemic adoption of in silico, in chemico, and in vitro diagnostic platforms.
The chemical landscape of high-throughput new approach methodologies for exposure | Science Inventory | US EPA
#whitepaper #safety-testing #translation
U.S. Environmental Protection Agency
Deploys the ExpoCast framework to integrate machine learning classifiers and non-targeted analytical screening, illustrating that alternative methodology models drastically broaden toxicokinetic coverage over historic animal testing pipelines.
The use of new approach methodologies (NAMs) to derive extrapolation factors and evaluate developmental neurotoxicity for human health risk assessment
#whitepaper #regulatory
U.S. Environmental Protection Agency
Technical case files submitted to the Scientific Advisory Panel detailing how in vitro data models can successfully replace standard inter- and intra-species uncertainty factors when assessing organophosphate pesticide risks.
Use of New Approach Methodologies
#whitepaper #safety-testing
U.S. Environmental Protection Agency
Evaluates the integration of advanced in silico profiling and high-throughput cellular screening frameworks designed to modernize and replace traditional animal metrics in the Endocrine Disruptor Screening Program.
EFSA - European Food Safety Authority #
An open European agency that evaluates and implements advanced non-animal methodologies, toxicokinetic modeling, and omics data integration to advance livestock and human food safety testing frameworks. [Link]
Brain Health Consortium presenting Advancing NAMs for Neurotoxicity
#whitepaper #safety-testing
European Food Safety Authority
Official proceedings and event registry detailing the joint strategic tracking and panel updates for scaling human-relevant alternative frameworks in developmental neurotoxicity mapping.
EFSA Pilot Project on New Approach Methodologies (NAMs) for Tebufenpyrad Risk Assessment. Part 1. Development of Physiologically-Based Kinetic (PBK) Model Coupled With Pulmonary and Dermal Exposure
#whitepaper #safety-testing
European Food Safety Authority
A comprehensive case study detailing the calculation of multi-route human exposure thresholds via automated computational biokinetic software models utilizing entirely non-animal data layers.
EFSA Pilot Project on New Approach Methodologies (NAMs) for Tebufenpyrad Risk Assessment. Part 2. Hazard characterisation and identification of the Reference Point
#whitepaper #safety-testing #regulatory
European Food Safety Authority
Outlines the second stage of the tebufenpyrad alternative pathway trial, demonstrating how quantitative points of departure can be isolated using alternative data streams to form regulatory chemical risk boundaries.
EFSA Project on the use of New Approach Methodologies (NAMs) for the hazard assessment of nanofibres. Lot 1, nanocellulose oral exposure: gastrointestinal digestion, nanofibres uptake and local effects
#whitepaper #safety-testing #invitro
European Food Safety Authority
Deploys an integrated tissue and assay approach (IATA) to systematically profile the metabolic breakdown, cellular absorption barriers, and localized tissue strain thresholds of ingested nanocellulose materials.
European stakeholders’ workshop on new approach methodologies (NAMs) for developmental neurotoxicity (DNT) and their use in the regulatory risk assessment of chemicals
#whitepaper #regulatory #translation
European Food Safety Authority
A prominent technical workshop report detailing the cross-sector validation criteria, diagnostic benchmarks, and collaborative guidelines used to move an international OECD alternative screening model for neurotoxicity into active draft implementation.
Exploring the use of Artificial Intelligence (AI) for extracting and integrating data obtained through New Approach Methodologies (NAMs) for chemical risk assessment
#whitepaper #safety-testing
European Food Safety Authority
Investigates the implementation of automated machine learning systems designed to crawl, parse, and systematically synthesize massive unstructured textual alternative methodology screening files into clear hazard assessment databases.
Maintenance, update and further development of EFSA’s Chemical Hazards: OpenFoodTox 2.0
#whitepaper #safety-testing #regulatory
European Food Safety Authority
Details structural data architecture upgrades to EFSA’s open database, expanding its processing capacity to catalog standardized non-animal parameter profiles, intermediate cellular effects, and harmonized international database formats.
Proposal for a qualification system for New Approach Methodologies (NAMs) in the food and feed sector: example of implementation for nanomaterial risk assessment
#whitepaper #regulatory #guidelines
European Food Safety Authority
Establishes a rigorous, step-by-step assessment protocol designed to systematically qualify and verify in vitro cellular screening assays and degradation parameters for formal safety submittals.
Review of New Approach Methodologies for Application in Risk Assessment of Nanoparticles in the Food and Feed Sector: Status and Challenges
#whitepaper #safety-testing #translation
European Food Safety Authority
An extensive technical synthesis profiling current nanoparticle-specific computational grids, chemical structure-activity predictions, and biological validation boundaries across the food sector.
Stakeholder Workshop on EFSA’s Genotoxicity Guidance Revision
#whitepaper #regulatory #guidelines
European Food Safety Authority
Official alignment brief and panel schedule detailing the programmatic revision of EFSA’s test protocols to support modern, animal-free mechanistic evaluation pathways.
TKPlate 1.0: An Open-access platform for toxicokinetic and toxicodynamic modelling of chemicals to implement new approach methodologies in chemical risk assessment
#whitepaper #safety-testing
European Food Safety Authority
A major landmark technical paper introducing an open computing environment that automates toxicokinetic and toxicodynamic simulations, enabling assessors to safely project internal dose dynamics and human clearance boundaries in silico.
User Guide for TKPlate 1.0: An open access platform for implementing new approach methodologies in chemical risk assessment through toxicokinetic and toxicodynamic modelling
#whitepaper #safety-testing
European Food Safety Authority
The authoritative software user manual defining data input formats, metabolic parameterization boundaries, and model configuration steps for running calculations inside the TKPlate environment.
Frontiers in Toxicology - Research Topics #
A peer-reviewed specialty portal detailing international breakthroughs in algorithmic modeling, molecular structural indexing, and genome-scale biochemical network simulators engineered to replace live vertebrate biological screens. [Link]
A New Immortalized Human Alveolar Epithelial Cell Model to Study Lung Injury and Toxicity on a Breathing Lung-On-Chip System
#research #safety-testing #bioprinting #chemical-testing
Arunima Sengupta, Nuria Roldan, Mirjam Kiener, … Olivier T. Guenat
Combines a human-derived immortalized alveolar epithelial cell line (${}^{AX}iAEC$) with advanced microfluidic organ-on-chip hardware to recreate breathing-like 3D cyclic stretch mechanical strains (10% linear strain, 0.2 Hz) at an air-liquid interface (ALI). Establishes a highly stable AT1/AT2 co-culture barrier (TER >1,000 $\Omega$ cm²) to evaluate inflammatory lipopolysaccharide responses, profibrotic TGF$\beta$1 pathways, and SARS-CoV-2 host factor expression without live animal models.
An Adverse Outcome Pathway for Decreased Lung Function Focusing on Mechanisms of Impaired Mucociliary Clearance Following Inhalation Exposure
#research #safety-testing #translation #chemical-testing
Karsta Luettich, Monita Sharma, Hasmik Yepiskoposyan, Damien Breheny, Frazer J. Lowe
Delineates a robust qualitative adverse outcome pathway (AOP) network on the AOP-Wiki mapping how chemical-induced oxidative stress causes down-regulation of CFTR and FOXJ1 proteins, leading to a cascade of airway surface liquid dehydration, ciliary beat frequency decay, and heightened mucus viscosity that results in chronic human lung function decline.
Application of Defined Approaches for Skin Sensitization to Agrochemical Products
#research #safety-testing #regulatory #chemical-testing
Judy Strickland, James Truax, Marco Corvaro, … Nicole Kleinstreuer
Tests 27 complex water- and solvent-based commercial agrochemical formulations across three non-animal assay platforms—DPRA (KE1), KeratinoSens (KE2), and h-CLAT (KE3)—to expand the applicability domain of OECD Guideline 497 defined approaches from monoconstituent substances to complex product mixtures.
Canadian Regulatory Perspective on Next Generation Risk Assessments for Pest Control Products and Industrial Chemicals
#whitepaper #regulatory #guidelines #chemical-testing
Yadvinder Bhuller, Deborah Ramsingh, Marc Beal, Sunil Kulkarni, Matthew Gagne, Tara S. Barton-Maclaren
Provides a detailed regulatory overview of Health Canada’s progressive integration of non-animal alternatives into its modern scientific review schemes, detailing weight-of-evidence (WoE) applications of in vitro transcriptomic points of departure, high-throughput bioactivity exposure ratios (BER), and multi-agency APCRA case studies to implement next-generation risk assessments.
Comprehensive genome-scale metabolic model of the human pathogen Cryptococcus neoformans: A platform for understanding pathogen metabolism and identifying new drug targets
#research #safety-testing #biometrics
Enes Fahri Tezcan, Yigit Demirtas, Zeynep Petek Cakar, Kutlu O. Ulgen
Presents “iCryptococcus,” the first comprehensive genome-scale metabolic simulator consisting of 1,270 reactions, 1,143 metabolites, and 649 genes mapped across eight computational cellular compartments. Validates network essentiality in silico to systematically expose active steroid and amino acid drug targets without executing live animal tissue assays.
Editorial: Chemical Testing Using New Approach Methodologies (NAMs)
#whitepaper #regulatory #translation #chemical-testing
Andreas O. Stucki, Amy J. Clippinger, Tala R. Henry, Carole Hirn, Todd J. Stedeford, Claire Terry
Introduces the special collection and provides an overarching technical baseline framing the progressive transition from legacy descriptive vertebrate assays toward human-relevant, mechanistically driven in vitro and in silico toxicity networks in alignment with the US National Research Council’s vision for 21st-century toxicology.
Evaluation of Inhalation Exposures and Potential Health Impacts of Ingredient Mixtures Using in vitro to in vivo Extrapolation
#research #safety-testing #regulatory #chemical-testing
Jingjie Zhang, Xiaoqing Chang, Tessa L. Holland, David E. Hines, Agnes L. Karmaus, Shannon Bell, K. Monica Lee
Deploys an in vitro to in vivo extrapolation (IVIVE) template using open-source physiologically based pharmacokinetic (PBPK) models (Gas_PBTK via the httk R package) to perform reverse dosimetry on e-cigarette aerosol mixtures. Models human equivalent administered doses (EADs) under three discrete parameters (single actor, additive effect, and outcome-oriented ingredient integration) to balance compound-specific absorption-clearance rates with overall toxicity tracking.
Molecular modeling, simulation and docking of Rv1250 protein from Mycobacterium tuberculosis
#research #safety-testing #biometrics
Sumita Choudhary, Anup Kumar Kesavan, Vijay Juneja, Sheetal Thakur
Deploys advanced automated structure prediction through the I-TASSER server alongside a 100-nanosecond molecular dynamics (MD) trajectory simulator to analyze steady-state static interactions between target bacterial transporter enzymes and prospective inhibitors in silico.
Human adenovirus DNA polymerase is evolutionarily and functionally associated with human telomerase reverse transcriptase based on in silico molecular characterization that implicate abacavir and zidovudine
#research #safety-testing #biometrics
Toluwase Hezekiah Fatoki
Applies deep computational molecular characterization, virtual target mapping, and molecular dynamics (MD) simulations to determine the binding affinity and thermodynamic stability of candidate antiviral chemical ligands, establishing a purely digital protocol for structural chemical evaluations.
Use of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes to Predict the Cardiotoxicity Potential of Next Generation Nicotine Products
#research #safety-testing #invitro #chemical-testing
Liam Simms, Fan Yu, Jessica Palmer, … Grant O’Connell
Utilizes human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) maintained via galactose media to transition cell bioenergetics from fetal glycolysis to adult oxidative phosphorylation. Leverages the metabolomics-based Cardio quickPredict assay to screen aqueous whole aerosol and smoke extracts via four core secretome markers (lactic acid, arachidonic acid, thymidine, and 2’-deoxycytidine) to reliably score functional cardiotoxicity potential in vitro.
Use of new approach methodologies (NAMs) to meet regulatory requirements for the assessment of industrial chemicals and pesticides for effects on human health
#whitepaper #regulatory #guidelines #chemical-testing
Andreas O. Stucki, Tara S. Barton-Maclaren, Yadvinder Bhuller, … Amy J. Clippinger
A comprehensive master review tracking the legal frameworks, intra-agency science initiatives, and operational flexibility clauses allowing for the deployment of alternative testing platforms across the US EPA (OPPT and OPP), US CPSC, Health Canada (HECSB and PMRA), ECHA, and EFSA.
Use of new approach methodologies (NAMs) to meet regulatory requirements for the assessment of tobacco and other nicotine-containing products
#whitepaper #regulatory #guidelines #chemical-testing
Jacqueline Miller-Holt, Holger P. Behrsing, Amy J. Clippinger, Carole Hirn, Todd J. Stedeford, Andreas O. Stucki
Analyzes the global legislative status of alternative test models under the US FDA Center for Tobacco Products (CTP) pre-market paths, Health Canada’s Tobacco Reporting Regulations, and the European Union’s Tobacco Products Directive (TPD). Recommends specific international intergovernmental frameworks and open data repositories to build institutional confidence in human-relevant in silico and organotypic testing workflows.
Versatile Functional Energy Metabolism Platform Working From Research to Patient: An Integrated View of Cell Bioenergetics
#research #safety-testing #invitro #chemical-testing
Sylvain Loric, Marc Conti
Deploys a clinical biochemical random-access multiparametric automation analyzer to continuously measure 22 critical homeostatic endpoints (including complexes I–V, glycolysis enzymes, and superoxide dismutase cascades) from a single 250 $\mu$L fluid sample. Restricts procedimiento coefficients of variation (CV%) below 5% to securely isolate subtle, low-noise chronic mitochondrial toxicities and cellular peroxidation in vitro and in vivo.
ICCVAM #
Interagency Coordinating Committee on the Validation of Alternative Methods A collaborative committee of federal regulatory and research agencies in the United States that establishes official roadmaps and validation metrics to transition federal bodies toward non-animal drug and safety evaluations. [Link]
A Strategic Roadmap for Establishing New Approaches to Evaluate the Safety of Chemicals and Products in the United States
#whitepaper #regulatory #guidelines
NICEATM
The landmark strategic planning framework detailing cross-agency directives, computational milestones, and public-private implementation plans engineered to phase out classic in vivo toxicity testing in favor of human-relevant predictive models.
Accelerating Adoption of NAMs with FAIR Principles
#research #regulatory
NICEATM
Examines specific repository case studies demonstrating how improving the findability, accessibility, interoperability, and reusability (FAIR) of public high-throughput data streams directly accelerates regulatory confidence and tool adoption.
Alternative Methods Accepted by US Agencies
#whitepaper #regulatory #guidelines
ICCVAM
An interactive, web-based reference tracking database configured to allow researchers and industry submitters to instantly cross-reference qualified non-animal testing methods against specific federal agency regulatory endpoints.
Annotations for ToxCast and Tox21 High-Throughput Screening Assays SOT 2023 Poster
#research #safety-testing
NICEATM
Details a massive curation effort to format, tag, and structurally annotate chemical bioactivity data from the ToxCast and Tox21 high-throughput screening libraries, transforming raw algorithmic files into easily interpretable regulatory profiles.
Application of Defined Approaches for Skin Sensitization for Agrochemical Products
#research #safety-testing #regulatory
NICEATM
Deploys fixed alternative data interpretation loops on commercial pesticide mixtures, demonstrating how non-animal testing strategies can accurately predict skin sensitization thresholds under active international guidelines.
Applying httk and In Vitro Data to Estimate Acute Neurotoxicity Potential
#research #safety-testing
USAF Predictive Risk Team
A joint computational case study detailing the application of the open-source httk R suite to map, perform reverse dosimetry, and run high-throughput chemical risk assessments on 220 potential neurotoxicants for the Department of Defense.
Applying In Silico Toxicity Models Across the Tox21 Chemical Space to Enhance DILI Predictivity
#research #safety-testing #biometrics
NICEATM
Deploys advanced structural informatics and deep learning algorithms across the entire Tox21 chemical catalog to significantly sharpen the in silico predictivity of drug-induced liver injury (DILI) parameters where legacy rodent studies fail.
Applying In Silico Toxicity Models Across the Tox21 Chemical Space SOT 2023 Poster
#research #safety-testing #biometrics
NICEATM
A technical presentation detailing the implementation of structure-based machine learning classifiers configured to screen massive compound lists for clinical liver hazard alerts.
Assay Application: Characterizing Sources of Variability in Non-Animal Methods
#whitepaper #safety-testing
ICCVAM
Investigates mechanical parameters, experimental bias vectors, and strict in-process control baselines required to neutralize noise and confirm the technical reproducibility of automated in chemico and in vitro screening assays.
Assay Development: Air-Liquid Interface (ALI) vs. Direct Liquid Systems
#whitepaper #safety-testing #invitro
ICCVAM
A performance evaluation study measuring data mapping parity between specialized cell exposures at an air-liquid interface (ALI) and conventional submerged liquid cultures, optimization benchmarks for respiratory toxicity NAMs.
Building Confidence in Alternative Methods Through ICE ASCCT 2021 Briefing
#research #safety-testing
NICEATM
Introduces the continuous user-interface upgrades and data integration tracks designed to expand the toxicological utility of the open Integrated Chemical Environment (ICE) portal.
Building Confidence in Alternative Methods Through ICE SOT 2022 Poster
#research #safety-testing
NICEATM
A technical poster illustrating how the query tools and curation pipelines configured inside ICE democratize access to validated alternative testing metrics for international risk managers.
Building Confidence in Alternative Methods Through ICE SOT 2022 Presentation
#research #safety-testing
NICEATM
Delineates how specific in vitro endpoints and model systems are compiled within the ICE infrastructure to serve as a reliable standalone alternative to live multi-organ mammal profiling.
Canadian Regulatory Perspective on Next Generation Risk Assessments for Pest Control Products and Industrial Chemicals
#whitepaper #regulatory #guidelines
Health Canada
Outlines Health Canada’s statutory work plans and weight-of-evidence (WoE) parameters to seamlessly deploy high-throughput exposure ratios and transcriptomic benchmarks instead of standard vertebrate toxicity tests.
Collection of Alternative Methods for Regulatory Application User Interface
#whitepaper #regulatory
NICEATM
A programmatic presentation revealing the structural database design of a public, interactive web dashboard crafted to let regulatory entities query qualified non-animal assay parameters filtered by exact context of use.
Combining NAM Data and IVIVE for Evaluating Potential Inhalation Toxicity SOT 2023 Abstract
#research #safety-testing #regulatory
NICEATM
Outlines a predictive template combining in vitro tissue data with open computational biokinetic packages to map target human equivalent exposure thresholds for inhaled environmental formulations.
Combining NAM Data and IVIVE for Evaluating Potential Inhalation Toxicity SOT 2023 Poster
#research #safety-testing #regulatory
NICEATM
Details a quantitative case study applying multi-compartment in vitro to in vivo extrapolation (IVIVE) methods to calculate reliable internal dose constraints, bypassing intact rodent lung assays.
Complement-ARIE: Complement Animal Research in Experimentation
#whitepaper #translation
NIH Office of Strategic Coordination
An authoritative presentation defining the mission objectives of the multi-million dollar NIH Complement-ARIE initiative, designed to systematically catalyze the standardization, scaling, and deployment of human-based alternative tools.
Curation of Eye and Skin Irritation Reference Data
#whitepaper #safety-testing
NICEATM
Documents a rigorous data curation strategy designed to measure and define the intrinsic historical variability of legacy rabbit skin and eye tests, establishing realistic target parameters for qualifying modern alternative assays.
Developing In Vitro Assay Annotations to Provide Context and Enhance Utility
#research #safety-testing
NICEATM
Presents a standardized data-annotation roadmap designed to tag raw in vitro screening files with precise mechanistic data layers, making large-scale chemical profiling sets instantly machine-readable for validation.
Development of a Tool for Integrating Traditional and New Approach Methodologies (NAMs) for Chemical Safety Decisions
#whitepaper #safety-testing #regulatory
NICEATM
Details the coding parameters of a regulatory decision platform that matches computational exposure analytics with cell-based points of departure to prioritize active compound files under TSCA timelines.
Engaging NAMs for Toxicant REgulation and Effects - ENTREE Project
#whitepaper #regulatory
ICCVAM
A technical progress update introducing the ENTREE computing engine, engineered to automate data ingestion from non-animal study submittals and benchmark their validation profiles against international guidelines.
Establishing Confidence in NAMs: Considering Variability in Reference Data
#research #safety-testing
NICEATM
An analytical report tracking how mathematical modeling of the baseline uncertainty and errors found in legacy animal testing sheets can prevent the false rejection of highly accurate non-animal algorithms.
Evaluation of substances of regulatory interest using non-animal test methods
#whitepaper #safety-testing #regulatory
NICEATM / Division of Translational Toxicology
A major technical evaluation report detailing the step-by-step screening metrics, minimum induction thresholds (MIT), and predictive performance bounds of advanced non-animal assays compiled on behalf of federal agency partners.
Evaluation of the GARDskin Sensitization Test Method Using Substances of Regulatory Interest
#whitepaper #safety-testing
NICEATM
A specialized peer-review data package evaluating the genomic-based GARDskin assay, documenting its capacity to classify chemical sensitization hazards by monitoring human transcriptomic biomarker profiles in vitro.
Facilitating NAM-Based Chemical Assessments with the Integrated Chemical Environment
#research #safety-testing
NICEATM
Introduces a newly integrated developmental toxicity query branch added to the ICE computational pipeline, connecting automated PBPK engines with non-animal tissue points of departure.
Formatting ToxCast and ICE cHTS Data Into OECD Reporting Templates
#whitepaper #regulatory #guidelines
ICCVAM
A high-value user framework mapping out the precise data formatting criteria required to package automated high-throughput screening metrics into internationally harmonized OECD harmonized templates.
GARD Models for Identification of Potential Skin and Respiratory Sensitizers
#whitepaper #safety-testing #invitro
ICCVAM
Reviews the mechanistic performance of the cell-based GARDair assay, validating an alternative testing template for respiratory hypersensitivity—an endpoint for which no reliable animal model exists.
Human-Based New Approach Methodologies for Biomedical Research | SACATM 2025 Presentation
#whitepaper #translation #regulatory
U.S. National Toxicology Program
An extensive advisory committee brief detailing strategic regulatory frameworks, reviewer training protocols, and programmatic hotlines designed to increase institutional confidence and publicize successful non-animal case validation pipelines.
Human Data for Skin Sensitization Method Evaluation Index
#whitepaper #safety-testing
NICEATM
The main repository link hosting highly vetted, clean human clinical datasets utilized by method developers to benchmark and verify the accuracy of upcoming dermal alternative algorithms.
Human data set for skin sensitization methods evaluation | ICCVAM Biennial Report 2023
#whitepaper #safety-testing
ICCVAM
Details a curated human reference dataset designed to systematically evaluate and score the predictive capacity, reproducibility, and biological accuracy of alternative in vitro and in silico skin sensitization assays.
ICE: Advancing Data Availability and Computational Tool Accessibility
#research #safety-testing
NICEATM
A comprehensive software architecture report summarizing recent updates to the ICE computing environment, designed to optimize large-scale user accessibility for multi-compartment risk screening tools.
ICE Tools to Support Chemical Evaluations SOT 2022 Poster
#research #safety-testing
NICEATM
A tactical reference walkthrough illustrating how data integration tools within the ICE ecosystem can be leveraged to harmonize raw in vitro screening outputs into standardized toxicity profiles.
ICCVAM Agency Activities
#whitepaper #regulatory #guidelines
ICCVAM
Outlines a permanent technical validation framework and comprehensive recommendations designed to guide pharmaceutical developers in substituting legacy rodent models with human-relevant cell-based arrays.
ICCVAM Biennial Progress Report 2020-2021
#whitepaper #regulatory
ICCVAM
A major statutory report detailing international agency alignment, technical testing program data streams, and specific strategic actions tracking the implementation of alternative endpoints into federal chemical policy.
ICCVAM Biennial Progress Report 2022-2023
#whitepaper #regulatory
ICCVAM
A comprehensive progress review documenting federal milestone metrics, quality management approaches, and strategic alignment parameters established to anchor scientific confidence in upcoming NAM frameworks.
ICCVAM Communities of Practice Webinars on (Developmental) Neurotoxicity
#whitepaper #safety-testing #translation
ICCVAM
Compiles the findings and inter-agency workshop briefs from the 2022 public forum, detailing strategic criteria for integrating microelectrode array data into regulatory decisions without mammalian tests.
ICCVAM Communities of Practice Webinars: Capturing the Leading Edge of NAMs
#whitepaper #translation
ICCVAM
A historical timeline presentation detailing agency-sponsored public learning modules (2018–2025) configured to build technical workforce literacy in toxicokinetic calculations and human-centric brain models.
ICCVAM Guidelines for Nomination and Submission of New, Revised, and Alternative Test Methods
#whitepaper #regulatory #guidelines
ICCVAM
The primary statutory submission manual establishing the technical requirements, biostatistical parameters, and performance criteria developers must satisfy to qualify a novel NAM for federal regulatory tracking.
ICCVAM Member Agencies Working Directory
#whitepaper #regulatory
ICCVAM
The central routing directory mapping specific chemical safety initiatives, training portals, and alternative method acceptance clauses across all 16 coordinating federal committee nodes.
ICCVAM Workgroup Update: ICCVAM Public Forum 2021
#whitepaper #regulatory
NICEATM
A technical committee report documenting baseline curation protocols for reference data sets, along with active testing models used to accelerate international legal validation pathways.
Improving Alternative Method Adoption Through Tools and Resources
#research #translation
NICEATM
An operational white paper reviewing the integration of international science policy efforts and public database tools required to successfully transition commercial laboratory protocols away from intact animal testing.
In Vitro Test Battery for Developmental Neurotoxicity Evaluation
#whitepaper #safety-testing #invitro
ICCVAM
Details an ongoing inter-agency evaluation program focused on microelectrode array platforms and neural connectivity software, optimizing automated alternatives for developmental toxicity screening.
In Vitro to In Vivo Extrapolation for Developmental Toxicity Risk Assessments
#research #safety-testing #regulatory
NICEATM
Evaluates automated pharmacokinetic modeling runs performed on 186 reference chemicals, establishing reverse dosimetry baselines to prove the reliability of in vitro alternatives in developmental toxicity filings.
Initiatives to Replace or Reduce Animal Use | ICCVAM Biennial Report 2023
#whitepaper #regulatory
ICCVAM
Examines systemic barriers, historical incentives, and strategic policy solutions required to transition legacy research portfolios away from established in vivo protocols toward non-animal testing strategies.
Integrated Chemical Environment: An Advanced Platform Aiding Alternative Method Development SOT 2023 Abstract
#research #safety-testing
NICEATM
Outlines software architecture criteria for seamlessly merging diverse cell-based datasets with predictive algorithmic grids, creating an accessible digital alternative to rodent testing layouts.
Integrated Chemical Environment: An Advanced Platform Aiding Alternative Method Development SOT 2023 Poster
#research #safety-testing
NICEATM
A technical poster demonstration showing how the PBPK, IVIVE, and chemical characterization modules inside ICE work in tandem to evaluate unknown compounds without animal tissue.
Integrating Bayesian Approaches with PBPK Modeling in a Human-Relevant Framework
#research #safety-testing #biometrics
NICEATM / NIEHS Workshop
Presents an advanced probabilistic framework that overlays Bayesian inference onto PBPK software templates, enabling automated mapping of population sensitivity margins via human in vitro parameters.
Integrating Screening-Level Developmental Neurotoxicity (DNT) Data Streams Into a Unified NAM Battery
#whitepaper #safety-testing #invitro
U.S. National Toxicology Program
A high-density strategic brief outlining how high-throughput cell-imaging data streams are linked into an interim screening matrix to reliably tag chemical developmental neurotoxicity risks.
Integrating Structure-Based Chemical Taxonomies to Focus Queries Across Large-Scale NAM Datasets WC13 Poster
#research #safety-testing #biometrics
NICEATM
Demonstrates the integration of automated ClassyFire structural chemical taxonomy classification scripts into open endpoints, a key mechanism to navigate high-volume data arrays generated by alternative workflows.
Integrating Structure-Based Chemical Taxonomies to Focus Queries Across Large-Scale NAM Datasets WC13 Presentation
#research #safety-testing #biometrics
NICEATM
An algorithmic report detailing data-mining pathways configured to group chemical structural families, minimizing query friction when searching thousands of automated in vitro bioactivity logs.
International Interactions | ICCVAM Biennial Report 2023
#whitepaper #regulatory
ICCVAM
Analyzes the global regulatory acceptance bottlenecks of modern NAM data streams and discusses harmonization approaches for new technologies where classical validation criteria are not applicable.
Investigating the Impact of Cytochrome P450 Metabolism on Chemical-Mediated Transcription Factor Activity
#research #safety-testing
NICEATM
Deploys a specialized in vitro metabolic activation overlay to capture how cytochrome P450 liver enzyme processing affects cell signaling, an essential technical requirement to replace rodent multi-organ clearance testing.
Methods2AOP: An International Collaboration to Integrate Assay Methods with Alternative Pathways
#research #safety-testing #translation
NICEATM
A collaborative technical report mapping a data infrastructure that automatically anchors discrete in vitro cell assay protocols to explicit molecular key events within the international Adverse Outcome Pathway framework.
Modernizing the Acute Toxicity ‘Six-Pack’ for U.S. EPA’s Office of Pesticide Programs
#whitepaper #regulatory #guidelines
EPA Office of Pesticide Programs
An official agency implementation brief documenting the specific criteria, policy rules, and testing guidelines deployed to substitute the classical in vivo “six-pack” animal tests with validated alternative datasets.
New Approach Methodologies for Use in FDA Food Safety Assessments
#whitepaper #safety-testing #regulatory
Suzanne Fitzpatrick, PhD, DABT
An official FDA forum briefing detailing the creation and operational parameters of the CFSAN NAMs working group configured to integrate predictive non-animal methodologies into standard food ingredient safety profiles.
New Interactive Database of Validated and Qualified NAMs for Regulatory Application
#whitepaper #regulatory
NICEATM / ICCVAM
A baseline committee overview introducing the design and public launch of an open-access interactive interface developed to help risk managers filter qualified alternative methods by regulatory authority.
NICEATM and ICCVAM Publications, 2022-2023
#whitepaper #translation
NICEATM
A curated bibliography tracking peer-reviewed publications and symposium abstracts focused on establishing the biological relevance of high-throughput alternatives and evaluating NAM configurations for tobacco harm reduction.
NICEATM Computational Tools and Resources Supporting Alternative Method Adoption
#research #safety-testing
NICEATM
An operational briefing surveying public computational architectures and data-sharing engines optimized to support both developer validation tracks and agency-level hazard evaluations.
NICEATM: Alternative Methods Main Portal Directory
#whitepaper #regulatory #guidelines
NICEATM
The main portal interface hosting public access tracks for the Integrated Chemical Environment (ICE), SEAZIT zebrafish evaluation data logs, and formal ICCVAM inter-agency strategy roadmaps.
OECD Guidance Document 34: Validation and International Acceptance of New or Updated Test Methods for Hazard Assessment
#whitepaper #regulatory #guidelines
Organisation for Economic Co-operation and Development
The foundation international text establishing standard scientific frameworks, performance testing criteria, and independent peer-review parameters required to declare a new testing methodology legally validated.
Policies and Guidance for Implementation of Alternative Methods | ICCVAM Biennial Report 2023
#whitepaper #regulatory #guidelines
ICCVAM
Documents specific policy updates and implementation tools deployed across member agencies to realize the operational mandates established by the 2018 ICCVAM Strategic Roadmap.
Presentation Abstracts: Using New Approach Methodologies to Address Variability and Susceptibility Across Populations
#whitepaper #safety-testing
NICEATM
Compiles high-density presentation synopses evaluating methodologies to model human population variability and genetic susceptibility thresholds using high-throughput in vitro cellular configurations.
Providing Context to In Vitro High-Throughput Screening Data Using ICE
#research #safety-testing
NICEATM
A high-density poster demonstration modeling how raw in vitro bioactivity data arrays from public libraries can be contextually mapped against baseline exposure boundaries to derive solid non-animal risk ratios.
Public Comment on ICCVAM Draft Report on Validation from PCRM
#whitepaper #regulatory
Physicians Committee for Responsible Medicine
A detailed advocacy comment submission offering precise parameter adjustments to ensure the updated ICCVAM validation framework is completely fit-for-purpose, fast-tracked, and focused on non-animal tech.
Public Comment on ICCVAM Draft Report on Validation from PETA Science Consortium
#whitepaper #regulatory
PETA Science Consortium International
A comprehensive technical feedback filing advising on training expansions and explicit communication workflows needed to actively build corporate and regulatory confidence in non-animal guidelines.
Publications About NICEATM & ICCVAM Activities in Scientific Journals
#whitepaper #translation
NICEATM
An indexed record tracking literature outcomes and agency perspectives regarding the landscape of alternative predictive methodologies submitted within modern regulatory drug filings.
Regulatory Applications of 3Rs
#whitepaper #regulatory #guidelines
NICEATM
An operational data portal mapping the specific regulatory contexts, test criteria, and agency-level parameters where the inclusion of non-animal data models is legally accepted or explicitly encouraged.
Roadmap Implementation Plans: Update on Each of the Acute Toxicity ‘Six-Pack’ Endpoints
#whitepaper #regulatory #guidelines
NICEATM / ICCVAM
A high-density strategic presentation reviewing the exact validation criteria, curation baselines, and cross-agency milestones achieved in replacing the classical animal “six-pack” testing endpoints.
SARA-ICE: Skin Sensitization Risk Assessment - Integrated Chemical Environment Module
#whitepaper #safety-testing
NICEATM
An operational hub introducing the SARA-ICE module, a predictive web tool that computes human-relevant skin allergy potency probabilities by combining defined approaches with machine learning.
Searches on Specific Alternatives Topics: MeSH Query Templates
#whitepaper #guidelines
NICEATM
Hosts a collection of optimized, high-density PubMed/MeSH medical search syntax strings designed to help researchers instantly filter out legacy vertebrate studies and isolate pure NAM literature.
Session 2 Presentation Abstracts and Background Materials: SACATM 2023
#whitepaper #safety-testing #translation
U.S. National Toxicology Program
A technical background review detailing cell-stress panels, computational toolsets, and in vitro models engineered to profile multi-individual population susceptibility without vertebrate testing.
The SARA-ICE Model for Predicting Quantitative Human-Relevant Skin Sensitizer Potency WC12 Abstract
#research #safety-testing
NICEATM
Details a mathematical weight-of-evidence (WoE) algorithm configured to integrate raw data inputs from in chemico assays and human cell arrays, returning precise human-relevant skin safety margins in silico.
The Skin Allergy Risk Assessment (SARA-ICE) Platform SOT 2023 Presentation
#research #safety-testing
NICEATM
A specialized case presentation demonstrating how the SARA-ICE module processes direct peptide reactivity data alongside cell-based logs (KeratinoSens/h-CLAT) to score toxicant potency.
Update on Alternatives Research at EPA Office of Research and Development
#whitepaper #safety-testing
EPA Office of Research and Development
A progress briefing reviewing computational milestones, microelectrode array validations, and multi-endpoint data modeling runs configured to expand the coverage of non-animal risk diagnostics.
User Guide for TKPlate 1.0 Coupled with Pulmonary and Dermal Exposure Distributions
#research #safety-testing #biometrics
European Food Safety Authority Case Studies
A high-density workshop slideshow explaining the integration of EFSA’s TKPlate environment with human cell-derived absorption data to accurately model pesticide clearance routes in silico.
Validation & Qualification of New Methods
#whitepaper #regulatory #guidelines
NICEATM
An authoritative developer guidance document defining a modernized framework for the validation, qualification, and swift regulatory acceptance of fit-for-purpose alternative methodologies.
Validation of the 21st Century Toxicology Toolbox: Historical Milestones and Systems Biology
#whitepaper #translation
NICEATM / Stokes et al.
A retrospective analysis detailing the structural transition of toxicological science from traditional animal endpoint tracking toward human-relevant systems biology networks and predictive computation.
Variability in Reference Test Method Data and the Impact on NAM Qualification SOT 2022 Poster
#research #safety-testing
NICEATM
Applies statistical modeling loops to standard laboratory animal data logs, demonstrating how measuring background noise in baseline animal responses prevents the false disqualification of alternative models.
Variability of In Vivo Toxicology Studies: Impact on NAM Evaluation WC12 Abstract
#research #safety-testing
NICEATM
Calculates the numerical ceiling of repeat-dose animal testing reproducibility, establishing that modern computational alternatives should be benchmarked against human relevance rather than legacy animal variations.
Lab on a Chip #
The premier international journal for microfluidic and nanofluidic research, hosting foundational papers on human organ-on-a-chip technologies designed to model human physiology and disease states. [Link]
3D printing monolithic, multifunctional polymer acoustofluidic devices with tunable mixing and particle focusing
#research #manufacturing #acoustofluidics
Balanay et al.
Demonstrates a simplified stereolithography approach to print unified, multi-feature polymer microchips capable of context-specific particle manipulation without complex multi-material bonding steps.
A 3D model to evaluate cell chemotaxis within a heterogenic tumor microenvironment
#research #organ-on-a-chip #cancer
Rodrigues et al.
Utilizes customized micro-chambers to replicate intercellular signaling gradients, shedding light on how tumors aggressively recruit supporting stromal structures within a local living architecture.
A 3D-printed electromagnetically actuated microgripper system for precision single-cell manipulation
#research #robotics #single-cell
Chen et al.
Introduces a miniaturized manipulation rig with a specialized liquid-retention barrier, offering non-destructive, highly isolated handling of target single cells.
A compact low-power valveless piezoelectric micropump with a nested rectification structure
#research #engineering #fluidics
Shan et al.
Details a specialized nested internal geometry designed to maximize forward flow velocity while significantly dropping energy demands for autonomous point-of-care chips.
A compact superlattice as a label-free surface-enhanced Raman scattering substrate for noninvasive urine testing for the diagnosis of lung cancer
#research #diagnostics #sensors
Zhang et al.
Applies a self-assembling crystal substrate to detect trace oncology biomarkers in patient fluid samples, benchmarking computational diagnostics against clean human metadata.
A customizable, low-cost 3D-printed device for live cell confinement imaging
#research #imaging #microscopy
Richman et al.
Presents an open-source, microscopy-compatible compression bracket providing highly uniform structural constraints for real-time observation of dynamic intracellular events.
A dual-antibody gold nanoparticle-based lateral flow assay for rapid and selective detection of mesenchymal stem cell stemness
#research #diagnostics #stem-cells
Prakashan et al.
Integrates mobile smartphone readout infrastructure with gold nanoparticle strips to allow high-purity, localized screening of functional cellular potency without legacy destroy-and-fix protocols.
A high-throughput liver-kidney metabolic interaction chip for insights into the nephrotoxicity mechanisms of triptolide
#research #organ-on-a-chip #toxicology
Liu et al.
Establishes a microfluidic co-culture network modeling metabolic interaction across interconnected tissue blocks, uncovering multi-organ downstream pathways without legacy animal dosing.
A microfluidic method for controlled generation and trapping of membraneless water-in-water droplets
#research #fluidics #droplets
Li et al.
Achieves high-stability generation of compartmentalized, open-system liquid arrays to enable complex biochemical processing inside localized dynamic micro-environments.
A microfluidic platform for automatic quantification of malaria parasite invasion under physiological flow conditions
#research #microfluidics #pathology
Kals et al.
Integrates targeted microchannels with automated processing engines to track real-time cellular invasion forces across human red blood systems subjected to realistic circulatory mechanics.
A microfluidic skin-on-a-chip enabling in situ construction of full-thickness human skin for modeling inflammatory diseases
#research #organ-on-a-chip #immunology
Sang et al.
Features a detachable architecture for long-term perfusion culture of realistic stratified human dermal constructs, allowing high-throughput drug screening against active multi-lineage inflammatory markers.
A novel 3D-printed tool for in vitro cell interaction studies under flow conditions
#research #methodology #cell-culture
Skoll et al.
Validates a highly accessible open platform named FlowCube that accurately introduces steady hydrodynamic shear forces to expose the structural blind spots of static petri-dish assays.
A portable, low-cost, point-of-care DNA amplification kit with impedance-based detection for decentralized antimicrobial resistance diagnostics
#research #diagnostics #sensors
Karimi et al.
Combines modified, enzyme-driven nucleic acid aggregation loop architectures with clean electronic impedance scanning for accurate multi-target identification out in decentral field environments.
A smart 3D microfluidic tumor spheroid-vessel co-culture model for studying exosomal HSP-mediated tumor invasion and angiogenesis
#research #organ-on-a-chip #cancer
Zhou et al.
Unifies vascular channels with multicellular tissue structures on a single chip to map down trace molecular signaling profiles responsible for initiating early vascular networks and metastatic movement.
A tumor spheroid array chip for high-fidelity evaluation of liposomal drug delivery through the EPR effect
#research #organ-on-a-chip #pharmacokinetics
Lee et al.
Models the actual mechanical interface of dense capillary matrices to validate and monitor the localized accumulative behavior of lipid nanoparticle therapeutics under systemic flow.
A wearable 3D-printed hollow microneedle device for pressure-driven interstitial fluid collection and testing
#research #wearables #diagnostics
Hacıosmanoğlu et al.
Introduces the μHolloSense architecture, integrating a matrix of fine fluidic needles with localized suction arrays for non-destructive, near-instant capture of clear human interstitial layers.
Acoustic probing of new biomarkers for rapid sickle cell disease screening
#research #diagnostics #acoustofluidics
Sridhar et al.
Employs precise surface acoustic wave vectors to rapidly evaluate mechanical red cell lysis variations, offering a quick, decentralized blood check format without legacy lab processing pipelines.
An AI-enabled tool for quantifying overlapping red blood cell sickling dynamics in microfluidic assays
#research #automation #imaging
Kadivar et al.
Trains a specialized nnU-Net mask layout integrated with down-line image segmentation to automatically analyze and score shape transformation metrics within crowded, unseparated cell fields.
An automated and portable platform for rapid cell-free DNA isolation and its application in microbial DNA metagenomic sequencing from human blood samples
#research #automation #genomics
Metcalf et al.
Details the CNASafe processing rig, which automatically isolates clean plasma targets to accelerate real-time portable nanopore library production and detect blood pathogenetic trends within hours.
An automated microfluidic system based on V-groove chip for rapid immunohistochemistry
#research #automation #diagnostics
Zhong et al.
Replaces conventional, slow human processing protocols with a V-shaped geometric chamber layout, compressing essential diagnostic sample profiling times down from hours to just 11 minutes.
An active-matrix digital microfluidic device based on surfactant-mediated electro-dewetting
#research #automation #fluidics
Xie et al.
Leverages a robust 4T2C layout array alongside surfactant chemistry adjustments to achieve fluid routing functionality under significantly lower input operating potentials.
An automated modular microfluidic platform for end-to-end mRNA synthesis and purification
#research #bioprocessing #automation
Sharma et al.
Unifies microscale synthesis nodes with sensor-led chromatography elements, establishing an optimized continuous fluid loop that boosts final pharmaceutical yields while dropping structural impurities.
Bacterial extracellular vesicles indirectly destabilize a human stem cell-derived blood–brain barrier on-chip through pro-inflammatory stimulation of immune cells
#research #organ-on-a-chip #immunology
Widom et al.
Applies an advanced multicellular barrier platform to prove that pathogenetic vesicle structures disrupt human neural line barriers through intermediary immune-line pathways rather than direct contact.
Band-stop microfluidics for high-purity, label-free enrichment of viable cancer cells from whole blood
#research #sorting #cancer
Krzeczkowski et al.
Uses a geometric microscale filter loop that isolates viable circulating tumor cell populations directly out of whole raw samples, allowing immediate downstream drug checks on the same substrate.
Bioinspired quality-based sperm sorting in a spiral microfilter-enhanced microfluidic device: enhancing DNA integrity via rheotaxis and boundary dynamics
#research #sorting #biomedical
Shahhoseini et al.
Employs boundary flow channels that sort highly functional reproductive populations by exploiting natural locomotion vectors, improving sample profile metric yields for clinical settings.
Cell therapy manufacturing at full clinical scale: enhancing the quality CAR-T cell therapy starting materials through massively parallel automated microfluidic cell sorting
#research #sorting #bioprocessing
Skelley et al.
Validates a massively parallelized microfluidic separation channel platform, processing real therapeutic batches to yield high leukocyte recoveries without initiating baseline cell activation stress.
Controlling spatial structure in minimal microbial communities by sequential capillary assembly
#research #microbiology #fluidics
Boggon et al.
Deploys capillaric force alignment patterns to precisely organize multiple microbial lineages into precise 2D structures, helping study local population dependencies with logical rigor.
Correction: Cell docking inside microwells within reversibly sealed microfluidic channels for fabricating multiphenotype cell arrays
#correction #microfluidics
Khademhosseini et al.
Amendments and textual validation notes linked to an engineered multi-phenotype cell array array template device.
Correction: A gut–brain axis on-a-chip platform for drug testing challenged with donepezil
#correction #organ-on-a-chip
Fanizza et al.
Formal structural correction dataset addressing multi-organ microphysiological axis connection parameters.
Digitally programmable microfluidic valving for autonomous, high-resolution continuous chromatographic purification
#research #automation #bioprocessing
Liao et al.
Coordinates single-use automated internal architecture nodes to carry out sequential buffer replacement, creating a robust path for long-term handling of delicate multi-protein targets.
Directed dielectrophoretic assembly and separation on microelectrodes patterned via stereolithography 3D-printed shadow masks
#research #manufacturing #electronics
Jo et al.
Uses low-cost printed mask sheets to achieve accurate, high-density substrate circuit mapping, validating robust particle collection setups without expensive cleanroom processing runs.
DROP-LCMS for wastewater surveillance of viral disease
#research #diagnostics #droplets
Peng et al.
Outlines a semi-automated, droplet-based microfluidic capture interface that directly couples regional source collection filters into liquid chromatography mass spectrometry systems for pathogenetic monitoring.
Dual vision-equipped microfluidic chip for spatiotemporal sequential pick-and-place of oocytes
#research #automation #robotics
Liang et al.
Integrates localized dual-angle camera networks with rapid micro-valve actuators to manage the high-precision routing of sensitive reproductive models across processing arrays.
Estimating single-cell elastic modulus in a serial microfluidic cytometer from time-of-flight and fluorescence signals analysis
#research #diagnostics #biophysics
Chickering et al.
Utilizes cell speed variations in specialized flow streams alongside optical readouts to infer precise individual membrane stiffness measurements without slow, mechanical probe configurations.
Facile fabrication of high-density two-dimensional micronozzle arrays using twisted thin-wire molds
#research #manufacturing #microfluidics
Takahashi et al.
Presents a simple mechanical routing method using skew-aligned fine wire templates to shape dense nozzle matrices for localized fluid delivery and cell processing arrays.
Fast and precise magnetophoresis of superparamagnetic nanoparticles on a micro-magnetic substrate in a static liquid environment
#research #magnetics #nanotechnology
Bou et al.
Details a specialized microscale magnetic array layout that drives target magnetic carriers across centimeter distances at speeds of 1.4 mm/s, accelerating downstream nucleic acid tracking.
Flow-programmable and reversible surface-induced LLPS in nanofluidic channels
#research #nanofluidics #biophysics
Ohta et al.
Exploits deep sub-micron structural confinement to initiate and sweep away localized phase-separated fluid layers, laying down rules to handle active targets inside nanofluidic loops.
Gut–liver-on-a-chip enables mechanistic study and risk assessment of drug-induced liver injury and drug–drug interactions
#research #organ-on-a-chip #toxicology
Yu et al.
Connects human intestinal and hepatic cell matrices inside a unified perfusion channel loop, offering a clear mathematical path to evaluate human drug absorption, clearance, and toxic metabolic dynamics.
High-speed liquid switching and on-chip force sensing reveal the transient mechanical response of MscL in Synechocystis sp. PCC 6803
#research #sensors #biophysics
Du et al.
Utilizes millisecond micro-actuator stream switching coupled with raw physical force monitors to track the immediate protective shape transitions of single living cells under osmotic changes.
High-throughput and efficient fabrication of engineered skeletal muscle tissue via streamlined 3D multimaterial bioprinting
#research #bioprinting #tissue-engineering
Lim et al.
Develops an multi-nozzle ink-delivery configuration that prints functional, aligned skeletal fiber blocks directly inside standard 96-well format layouts, simplifying large chemical libraries screening.
High-throughput label-free assessment of sperm DNA fragmentation index via intelligent morphological imaging
#research #imaging #automation
Jin et al.
Combines structural microscale flow channels with rapid camera capture loops and trained morphological scoring systems to identify genetic core health features without destructive tag dyes.
High-throughput single-cell proteomics and transcriptomics from same cells with a nanoliter-scale, spin-transfer approach
#research #automation #genomics
Dawar et al.
Deploys a stacked nanoliter-scale fluidic transfer plate matrix that segregates and processes single-cell lysate components to output matched, high-resolution protein and transcript maps from individual source models.
High-throughput microfluidic platform for modelling inflammatory responses of human articular chondrocytes under variable fluid shear stress
#research #organ-on-a-chip #pathology
da Silva et al.
Presents a scalable, pumpless structural format that uses continuous gradient streams to match and reproduce the complex arthritic joint changes found under pathogenetic mechanical load.
Human hair regeneration using organoids and hair-on-chip technologies
#research #tissue-engineering #organoids
Ahmed et al.
Highlights critical biofabrication design tracks merging advanced multi-lineage skin microphysiological structures with localized fluidics, aiming for complex tissue replacement models without human-to-animal grafting loops.
Hydrogel microwell with pneumatic soft actuator for compression formation of three-dimensional cellular tissue
#research #tissue-engineering #biomaterials
Kawamae et al.
Combines soft flexible elastomer pressure cells with patterned gel array matrices, forcing individual cell clusters into structured, highly repeatable 3D architecture setups under defined load.
In vitro space of Disse model for exploration of drug induced hepatotoxicity
#research #organ-on-a-chip #toxicology
Mesic et al.
Recapitulates complex liver capillary architecture by matching porous gel matrices with functional endothelial and parenchymal human cell lines to trace out cellular breakdown mechanisms during toxic pharmaceutical compound exposure.
Inkube: an all-in-one solution for neuron culturing, electrophysiology, and fluidic exchange
#research #open-source #neuroscience
Maurer et al.
Details an open-source hardware system combining continuous multi-channel signal arrays with automated microscale fluid routing to track functional human neural layer activity under steady long-term observation.
Integrated microfluidic platform based on potentiometric Sonogel-Carbon sensors for the simultaneous determination of Na+ and K+ in untreated human plasma and serum
#research #sensors #diagnostics
Sainz-Calvo et al.
Combines precise carbon sensor matrices directly into raw fluid pathways to run immediate, simultaneous tracking of critical ion concentrations without preparatory sample filtering steps.
Integrated microfluidic platform for inertial separation and encapsulation of single cells in droplets
#research #sorting #droplets
Galogahi et al.
Unifies physical size-sorting channels with droplet generation nozzles on a single substrate chip, providing a stream that cleans, routes, and packages individual cells for downstream processing.
Integrated strategy for breast cancer biomarker analysis using dual ionic liquid aqueous biphasic systems and microfluidic immunoassays
#research #diagnostics #sensors
Mendes et al.
Employs an internal multi-phase fluid partitioning layout that selectively strips high-abundance protein background noise out of samples, elevating downstream target biomarker read resolution.
Intelligent image-activated sorting of large cells enabled by elasto-inertial focusing
#research #sorting #automation
Nagasaka et al.
Integrates long, non-turbulent elasto-inertial guiding channels with real-time deep learning layout logic to achieve rapid, content-driven classification of large models under high-throughput conditions.
Intensified lentiviral vector perfusion bioprocessing with a spiral inertial microfluidic cell retention device
#research #bioprocessing #fluidics
Bevacqua et al.
Deploys high-velocity curved channels to keep production cell masses separated from output streams via inertial vectors, achieving steady high-density vector harvest outputs over long run durations.
Label-free assessment of a microfluidic vessel-on-chip model with visible-light optical tomography reveals structural changes in vascular networks
#research #imaging #microfluidics
Veerman et al.
Applies visible-light optical tomography inside structural microphysiological chips, providing clear, deep 3D structural mapping of human vascular networks without utilizing destructive tracking dyes.
Label-free monitoring of therapy response in 3D spheroids using lab-on-a-chip impedance spectroscopy
#research #sensors #cancer
Macke et al.
Deploys real-time multi-frequency electrical scanning channels around living tissue spheroid blocks, providing an objective mathematical readout track for recording oncology model radiation responses.
Lyocell–modal thread microfluidic platform integrated with a microneedle sensor for lactate detection in saliva
#research #sensors #wearables
Ding et al.
Combines processed cellulose fiber paths with localized electrochemical tip sensors, constructing a practical fluid-guiding lane for immediate, non-invasive assessment of human sample chemical metrics.
Machine learning-augmented lateral flow assays for point-of-care infectious disease diagnostics
#research #diagnostics #automation
Parmaksizoglu et al.
Reviews progress across nanomaterial paper diagnostics coupled with computer vision software, outlining a path toward objective smartphone-driven screening out in remote clinic networks.
Machine learning-driven single-cell phenotyping in size-controlled microenvironments via parallel deterministic droplet microfluidics
#research #automation #droplets
Lee et al.
Encapsulates individual target profiles across a range of calibrated matrix environments, utilizing neural network classifier systems to automatically analyze cell responses under physical confinement.
Macroporous transport – mesoporous catalysis: a rapid microfluidic-fabricated biomimetic sponge photocatalytic microsphere reactor
#research #chemical-engineering #manufacturing
Wang et al.
Assembles biomimetic multi-scale catalytic microspheres inside high-precision droplet streams, providing an optimized layout that enhances substrate access and reaction efficiency.
MaGIC-OT: an AI-guided optical tweezers platform for autonomous single-cell isolation in microfluidic devices
#research #automation #robotics
Cieslik et al.
Combines active computer vision tracking with optical trapping nodes to safely navigate rare single target bodies out of highly crowded field environments without manual control.
Miniaturisation of Raman spectroscopy systems: from benchtop to backpocket
#research #sensors #spectroscopy
Hardy et al.
Traces the optical integration steps and component innovations that compressed traditional bulky benchtop laser analysis rigs down into pocket-sized solid-state field units.
Monolithic 3D-printed split-and-recombine micromixer integrated into a microfluidic concentration gradient generator
#research #manufacturing #microfluidics
Navarro Molina et al.
Validates a unified single-print fluidic device containing complex internal routing channels that guarantee thorough stream mixing across an exceptionally wide range of flow volumes.
Multiplexed nanophotonic biosensing and deep learning-driven protein quantification for traumatic brain injury diagnosis at the point of care
#research #diagnostics #sensors
Liu et al.
Deploys a specialized chip containing nanophotonic sensing nodes backed by deep learning interpretation models, generating clean multi-biomarker score outputs from blood drops within minutes.
Multiphasic droplet microfluidics platform for controlled bacteria and mammalian cell co-culture
#research #droplets #microbiology
Alshareedah et al.
Exploits aqueous polymer phase boundary structures inside micro-droplet arrays to culture animal lines and bacterial systems side by side, avoiding early overgrowth issues.
Muscle regeneration on a chip: exercise-induced microtrauma and optimal mechanical stimulation regimen
#research #organ-on-a-chip #biophysics
Yin et al.
Employs flat flexible substrate structures to introduce precisely controlled cyclic tensile strains across mature human cell models, mapping out structural tissue repair responses.
MyeliMAP: a microfluidic-multielectrode array hybrid platform to investigate oligodendrocyte function in human iPSC derived brain-like networks
#research #organ-on-a-chip #neuroscience
Ahuja et al.
Unifies high-density multielectrode processing arrays with precise human cellular channels to track axonal conduction velocity variations and check functional chemical line interactions.
Nanomembrane-based microfluidic platform with embedded electrical pressure transducer for on-chip nanoparticle quantification
#research #sensors #nanotechnology
Morris et al.
Monitors exact physical resistance variations across an internal nanoporous membrane sheet, using a predictive algorithm to infer target concentration matrices from fluid dynamics change.
Numerical microfluidic chip modeling of laminar vortex dynamics induced by biomineralization in evolving porous media
#research #fluidics #physics
Chu et al.
Develops detailed 3D fluidic simulations to trace how mineral precipitation profiles alter local path layouts, demonstrating the formation of internal vortex nodes that alter chemical mixing.
On cloud microfluidic experiment platform powered by in situ maskless lithography
#research #automation #methodology
Paul et al.
Details a remote cloud computing platform connected to an automated digital optical pattern projector setup, enabling users to submit chip designs and track experiments online.
On-chip acoustic chaotic micromixer for point-of-care applications
#research #fluidics #sensors
Chen et al.
Presents a compact fluid processing layout driven by a surface acoustic Lamb wave resonator grid, executing continuous extraction, rapid sample preparation, and detection steps on a single board.
Oscillatory flow for contactless particle trapping
#research #microfluidics #physics
Saint-Girons et al.
Deploys specialized microchannel geometric structures to manipulate steady oscillating fluid columns, generating stable spatial collection nodes that retain delicate targets without solid contact.
PDMS aqueous leachates cause acute toxicity in C. elegans
#research #toxicology #materials
Gomez et al.
Investigates chemical safety limitations across standard microfluidic substrate materials, proving that un-cleared internal polymer leachates trigger acute system stress pathways within simple living formats.
Point of care molecular cancer diagnostics
#research #diagnostics #methodology
Bhaskar et al.
Outlines a unified translation framework for pocket-sized oncology screeners, linking patient clinical benchmarks directly with recent advancements in microfluidic sensor integrations.
Point-of-care SERS platforms: integrating microfluidics and machine learning for disease screening
#research #diagnostics #sensors
Chen et al.
Reviews how advanced plasmonic metal substrates coupled with automated fluid handling and pattern recognition algorithms boost the reliability of fast, laser-driven patient bio-screening.
Polydiacetylene (PDA) coated paper-based fluorescence sensor for the detection and quantification of bisphenol
#research #sensors #chemical-safety
Loganathan et al.
Develops an un-tagged, paper-based diagnostic sheet coated with a responsive polymer compound matrix that yields specific optical shifts when exposed to toxic consumer bpa strains.
Porous microneedle-based electrochemical aptamer biosensor for the collection and quantitative analysis of dry eye disease biomarkers
#research #diagnostics #wearables
Ko et al.
Combines porous liquid-drawing structure elements with surface-bound aptamer circuit lines to carry out direct sampling and targeted diagnostic profiling on a single platform.
Predicting human pharmacokinetic parameters of drugs using a multi-tissue chip platform integrating liver, kidney, and skeletal muscle microphysiological systems
#research #organ-on-a-chip #toxicology
Sherfey et al.
Integrates human liver, kidney, and skeletal fiber microphysiological blocks with deep quantitative systems pharmacology logic, generating precise human metabolic parameter models without using animals.
Rapid desiccation and on-disc rehydration of extracellular vesicles for non-cryogenic preservation
#research #methodology #bioprocessing
Woo et al.
Preserves cellular target vesicles via a centrifugal spin layout that manages low-temperature water extraction, avoiding the high cost and structural damage of legacy liquid nitrogen storage lines.
Real-time high-throughput characterisation of the surface elasticity of suspended cells
#research #imaging #biophysics
Guo et al.
Links continuous microscale deformation channels with fast tracking engines and biophysical stress formulas, outputting objective stiffness profiles for large collections of individual cells.
Real-time impedance-based cell migration measurements with integrated electrodes on porous membranes for next generation microphysiological systems
#research #sensors #microfluidics
Torres-Castro et al.
Deposits ultra-thin electrode tracking matrices directly onto both faces of a track-etched membrane core, allowing ongoing monitoring of cell movement across the barrier layer.
Region-specific proteomic profiling of brain interstitial fluid via a micro-invasive sampling platform
#research #neuroscience #methodology
Cao et al.
Deploys a fine, membrane-free internal sampling tool to collect un-diluted neural fluid volumes, uncovering specific regional protein differences that conventional crude processing misses.
Robotic acoustofluidic single-cell picking and placement platform
#research #automation #robotics
Li et al.
Unifies localized high-frequency sound wave drivers with automated stage movements to handle single microscale targets without damaging structural features.
Size-based sorting of cancer cells reveals functional heterogeneity among subpopulations
#research #sorting #cancer
Yilmaz et al.
Employs clean deterministic lateral displacement post arrays to slice up tumor lines into precise size pools, revealing clear differences in adhesion and local migration speed metrics.
Size-based sorting of dynamic bacterial clusters
#research #sorting #microbiology
Akbari et al.
Tracks how deformable streptococcal aggregate chains pass through high-precision micro-post networks, setting out rules to safely isolate heterogeneous clinical profiles under steady stream flow.
Smarter cell sorting: droplet microfluidics meets pick-and-place sorting
#research #sorting #automation
Gerhard et al.
Combines continuous multi-phase stream routing blocks with a precise microscale positioning tip tool to successfully package and move individual target structures into a clean droplet line.
Stretchable mesoporous electrodes as a versatile platform for minimally invasive surgical devices
#research #engineering #biomedical
Listyawan et al.
Integrates thin, highly flexible metal mesh layers onto soft surgical guide structures, enabling multi-point bio-potential and tracking functionality inside variable physical structures.
Stroke volume analog on a chip – in vitro hydrodynamic model of cardiac pumping efficiency
#research #organ-on-a-chip #cardiology
Zimmerman et al.
Maintains contractible human tissue films inside a precise microscale chamber to evaluate stroke performance profiles under real chemical dosing, expanding alternatives to animal model trials.
Systematic characterization and mechanistic insights into ultrasonically actuated sharp-tip capillary droplet generation
#research #fluidics #manufacturing
Zhang et al.
Details the specific tip oscillation dynamics used to split streams into multi-volume droplet groups without requiring conventional etched chip shapes or high-pressure pump setups.
Systematic investigation of double emulsion dewetting dynamics for the robust production of giant unilamellar vesicles
#research #biophysics #nanotechnology
Jing et al.
Constructs a physics-led fluidic framework mapping out double-emulsion structural changes, giving researchers a reliable recipe to manufacture stable, clean synthetic cell profiles.
Thin stencil membrane-assisted high throughput single-cell to cluster of cells micropatterning and large-size biomolecular transfection in primary and stem cells
#research #manufacturing #biomedical
Dominic et al.
Uses thin, laser-patterned carbon mesh sheets to anchor individual cells before running pulsed laser photoporation lines, achieving highly efficient macromolecular cargo delivery.
Topology-based coordination control for multi-droplet tasks in autonomous digital microfluidics
#research #automation #fluidics
Guo et al.
Coordinates large matrix arrays with instant computer vision loop analysis, maintaining precise tracking and independent routing of multiple fluid units simultaneously across open boards.
TopoChip-based high-throughput screening of micropatterned hydroxyapatite to guide stem cell behavior and accelerate bone regeneration
#research #biomaterials #tissue-engineering
Li et al.
Profiles cellular responses across 127 individual surface layout designs stamped onto ceramic substrates, identifying specific groove setups that accelerate bone matrix formation.
Tumor-on-chip platforms for transport phenotyping: decoding CAF-driven barriers to drug delivery
#research #organ-on-a-chip #cancer
Le Manach et al.
Constructs live 3D oncology matrices to map out how cancer-associated fibroblasts remodel local gel frameworks, creating measurable physical barriers that drop therapeutic chemical delivery.
Tunable squeeze-activated GHz acoustofluidics for stable trapping and separation of sub-100 nm nanoparticles
#research #nanotechnology #fluidics
Liu et al.
Deploys a localized gigahertz acoustic driver line to create stable energy troughs in microfluidic streams, separating and trapping particle profiles down to 50 nm scale lengths.
Two-phase simulations of viscoplastic flow in superhydrophobic microchannels: interface stability, plug dynamics, and drag reduction
#research #fluidics #physics
Joulaei et al.
Builds explicit fluid equations tracing how non-newtonian thick media moves past textured internal microscale ridges, providing a clean path to minimize pressure limits inside analytical channels.
Ultra-high throughput droplet microfluidics for cultivation and functional screening of environmental microbial strains and consortia
#research #sorting #microbiology
Potenza et al.
Outlines a reliable microfluidic loop that isolates un-cultured local environment cells inside protected water-in-oil droplet pools, expanding screening speeds for functional bioprospecting.
Valved microfluidics with Ostemers
#research #manufacturing #materials
Kumar et al.
Replaces conventional high-maintenance elastomer structures with stable three-layer polymer molds, achieving crisp 200 ms channel stream gating under aggressive chemical settings.
Vascularizing organoids-on-chip for perfused and personalized models
#research #organ-on-a-chip #tissue-engineering
Menzani et al.
Reviews technical design strategies used to grow and weave capillary loops straight through mature human organoid bodies, pushing up long-term model realism and utility for personal diagnostics.
Vector-free DNA transfection by nuclear envelope mechanoporation
#research #biomedical #genetics
Akh et al.
Deploys microscale wedge channels to induce precise, brief mechanical stretching states across cell walls, allowing large target plasmid targets to migrate cleanly into nucleus structures.
Vertical numbering-up microfluidic architecture for scalable and homogeneous lipid nanoparticle production
#research #bioprocessing #manufacturing
Zhang et al.
Arranges precision mixing channel matrices in a stacked three-dimensional block layer layout, multiplying therapeutic nanoparticle production limits while maintaining tight output particle metric bounds.
WAFFLE – an automated platform for enhancing the performance of electrochemical biosensors
#research #sensors #automation
Dobrea et al.
Combines an automated physical handling rig with open analytical processing software scripts, reducing error rates across microscale bio-sensing array validation runs.
NAM Journal #
An open-access publication which serves as a hub for dissemination and worldwide exchange of information regarding state-of-the-art NAM developments. [link]
An in vitro investigation of diet-influenced gut bacterial metabolites on the onset of Type 2 diabetes mellitus using a multi-cellular human primary islet model
#research #metabolism #diabetes
Guraka et al.
Utilizes a primary islet cell system to study the link between gut-derived metabolites and insulin signaling pathways, identifying potential metabolic disruption triggers associated with dietary changes.
A novel in vitro alveolar model (ALIsens®) for hazard assessment of methyl methacrylate: No evidence for respiratory sensitisation potential
#research #safety-testing #respiratory
Gutleb et al.
Validates the ALIsens® lung-on-a-chip platform for assessing respiratory sensitization, successfully clearing methyl methacrylate of sensitizing hazard using human-relevant cellular exposure interfaces.
A sociological perspective on the challenges of displacing animal research within academia: the contribution of Bourdieu
#reviews #policy #sociology
Pandora Pound
Applies Bourdieusian field theory to map out the entrenched cultural mechanisms, institutional funding loops, and academic inertia that slow the transition from legacy animal lines to alternative non-animal architectures.
Characterizing common loss-of-function genes and their potential utility in assessing population variability and chemical susceptibility in vitro
#research #genomics #susceptibility
Chanhee Kim et al.
Leverages human engineered knockout cell cohorts to evaluate internal genetic variations, standardizing a human cell-based blueprint to map chemical susceptibility swings across diverse populations without animal testing.
From concept to context-of-use: A framework for Organ-on-Chip development
#protocols #policy #organ-on-a-chip
Araújo-Gomes et al.
Outlines a systematic engineering and validation pipeline to bridge the gap between academic chip prototyping and formalized, reproducible contexts-of-use required for commercial pharma uptake.
Investigations into the biological activity of avobenzone as part of a Next Generation Risk Assessment
#research #safety-testing #dermatology
Shan et al.
Deploys a robust Next Generation Risk Assessment (NGRA) tier to evaluate the bioactivity profiles of the sunscreen agent avobenzone, ensuring high-confidence human safety profiling without legacy animal models.
NAMazing: The importance of being earnest - a 2025 cross-Atlantic turning point in phasing out animal testing
#editorial #policy #safety-testing
Hansell & Hartung
Reflects on the pivotal 2025 shifts in US and European regulatory landscapes, emphasizing the urgent need for a unified, sincere commitment to finalizing the phase-out of animal-based toxicological protocols.
Never-ending Acronym Madness: Proposing a harmonized definition for NAMs
#editorial #terminology #policy
LaFollette et al.
Critiques the terminological inflation surrounding “New Approach Methodologies,” proposing a rigorous, harmonized definition to improve consistency and communication within regulatory and scientific sectors.
Set up of a human 3D liver multicellular model for chemical high-throughput toxic hazard assessment
#research #safety-testing #hepatology
Recoules & Audebert
Details the creation of a 3D multi-cell hepatic platform optimized for high-throughput screening, enabling rapid, multi-lineage assessment of chemical-induced liver damage.
SILIFOOD: a tool to support the risk assessment of non-evaluated food contact material substances
#research #automation #food-safety
Streel et al.
Introduces the SILIFOOD computational tool, designed to streamline the hazard characterization of chemical substances in food contact materials that currently lack exhaustive toxicological evaluation.
Nature Biomedical Engineering #
A high-impact journal publishing peer-reviewed research on technologies that combine engineering with the life sciences, serving as a primary outlet for advanced microphysiological systems (MPS) and organ-on-a-chip developments. [Link]
3D biofabricated in vitro models as new approach methodologies for animal alternatives
#research #safety-testing #bioprinting
Hua & Gaharwar
Evaluates recent progress in 3D-bioprinted constructs and advanced cell matrix platforms acting as alternatives to traditional animal pipelines, analyzing current readiness boundaries for clinical drug verification.
A predictive in vitro risk assessment platform for pro-arrhythmic toxicity using human 3D cardiac microtissues
#research #safety-testing #cardiology
Kofron et al.
Deploys human-derived three-dimensional myocardial syncytia arrays to construct a highly sensitive electrical scoring baseline, demonstrating a robust human cell-based alternative for preclinical cardiotoxicity screening.
An engineered IL-2 reprogrammed for anti-tumor therapy using a semi-synthetic organism
#research #biotechnology #immunology
Ptacin et al.
Utilizes an expanded genetic code inside synthetic living structures to execute site-specific chemical modifications on interleukin-2, generating stable, high-efficiency oncology immunotherapies.
Applying single-cell and single-nucleus genomics to studies of cellular heterogeneity and cell fate transitions in the nervous system
#reviews #neuroscience #genomics
Adameyko et al.
Outlines rigorous multi-omic study design tracks for building comprehensive cell atlases, capturing discrete transitional transcript boundaries across developing or pathogenetic neural architectures.
Chemically-defined medium formulation and adaptation method for supporting growth of endothelial cells
#research #cell-culture #methodology
Brunmaier & Walker
Formulates a completely animal-free, chemically defined fluid standard to support continuous microvascular line growth, eliminating un-standardized serum components from microphysiological systems.
eUnaG: a new ligand-inducible fluorescent reporter to detect drug transporter activity in live cells
#research #imaging #pharmacology
Yeh et al.
Validates a highly responsive, non-destructive fluorescent marker platform to track clear chemical efflux mechanics inside live cell configurations in real time.
Generation of human brain region–specific organoids using a miniaturized spinning bioreactor
#protocols #organ-on-a-chip #neuroscience
Qian et al.
Provides step-by-step procedures to build the SpinΩ engineering platform, directing human stem cell lines toward highly differentiated structures that closely mirror native human forebrain, midbrain, and hypothalamic spatial development.
Immune-competent new approach methodologies for a hybrid future
#reviews #safety-testing #immunology
Soares et al.
Advocates for the systematic integration of complex human immune line networks into reductionist organ-on-chip arrays, establishing a sophisticated hybrid environment necessary for matching true human clinical outcomes.
Integrating genetic and non-genetic determinants of cancer evolution by single-cell multi-omics
#reviews #cancer #genomics
Nam et al.
Examines single-cell multi-omic profiling platforms that simultaneously sequence heritable genetic and non-genetic mutations, mapping out how local environment interactions drive complex tumor evolution.
Meso–macroporous hydrogel for direct litre-scale isolation of extracellular vesicles
#research #nanotechnology #manufacturing
Kim et al.
Details a cryo-photocrosslinked porous gel matrix that carries out scalable extraction of delicate diagnostic vesicle targets from massive sample streams while preserving structural metadata.
Multi-omics analyses reveal regulatory networks underpinning metabolite biosynthesis in Nicotiana tabacum
#research #genomics #systems-biology
Li et al.
Applies multi-layered computational models to trace the underlying transcriptional hubs and regulatory network structures that govern metabolic synthesis inside complex plant chassis.
Quantitative assessment of sensitizing potency using a dose–response adaptation of GARDskin
#research #safety-testing #toxicology
Gradin et al.
Implements an advanced human cell line genomic screening format that leverages specific transcript marker responses to mathematically calculate allergen potency variations without legacy animal skin exposure.
Towards in vitro models for reducing or replacing the use of animals in drug testing
#reviews #safety-testing #organ-on-a-chip
Stresser et al.
Examines the pharmaceutical sector’s advocacy for microphysiological systems to replace traditional animal pipelines, analyzing the operational and biological advancements required to heighten model readiness for regulatory entry.
Unlocking gene regulatory networks for crop resilience and sustainable agriculture
#reviews #genomics #systems-biology
Leong et al.
Reviews targeted transcriptomic modeling tracks mapping out plant network structures, identifying genetic hub targets to accelerate sustainable engineering programs.
OECD #
Organisation for Economic Co-operation and Development An intergovernmental organization that publishes internationally agreed-upon guidelines, white papers, and testing standards that form the global legal bedrock for cross-border regulatory acceptance of non-animal safety methods. [Link]
Case Studies for the Integrated Approaches for Testing and Assessment (IATA) | OECD
#research #safety-testing #iata
OECD
Compiles recent regulatory initiatives across the US EPA, Environment and Climate Change Canada, and ECHA, focusing on structural implementations of multi-evidence data architectures to optimize human chemical risk classification models.
Case Study on use of an Integrated Approach to Testing and Assessment (IATA) | OECD
#research #safety-testing #iata
OECD
Establishes a foundational proof-of-concept for read-across workflows, leveraging a unified structure of computational data, in vitro toxicodynamics, and toxicogenomics to quantify biological similarity without traditional in vivo testing.
Cross Country Analysis: Approaches to Support Alternatives Assessment | OECD
#reviews #safety-testing #policy
OECD
Provides a multi-national comparative analysis on the structural replacement of endocrine-disrupting variables like plasticizers and flame retardants, utilizing innovative computational paradigms to standardize exposure maps.
Guidance on Good Practices and Standardisation of Sample Collection for Omics Technologies | OECD
#protocols #toxicology #genomics
OECD
Outlines rigorous structural harmonization rules for sample collection and metadata management in regulatory transcriptomics, defining standardized molecular points-of-departure for chemical grouping.
Guidance on Grouping of Chemicals, Third Edition | OECD
#protocols #safety-testing #read-across
OECD
Presents the definitive regulatory framework for establishing chemical category logic and chemical similarity protocols, incorporating mechanism-based methodologies including omics and adverse outcome pathways (AOPs) to bridge non-animal data gaps.
Overview of Concepts and Available Guidance related to Integrated Approaches to Testing and Assessment (IATA) | OECD
#reviews #safety-testing #iata
OECD
Examines the definition boundaries and baseline prescriptive parameters governing international testing methods, aiming to harmonize cross-border data acceptance frameworks for animal-free data matrices.
Workshop Proceedings on Critical Innovations in Pesticides Safety | OECD
#research #toxicology #neuroscience
OECD
Details strategic regulatory pathways for embedding alternative, human cell-based methodologies into developmental neurotoxicity (DNT) risk evaluations, focusing on modern pesticide evaluation architectures.
Regulatory Toxicology and Pharmacology #
A monthly peer-reviewed scientific journal which covers legal aspects of toxicological and pharmacological regulations. [Link]
A 10-step framework for use of read-across (RAX) in next generation risk assessment (NGRA) for cosmetics safety assessment
#protocols #safety-testing #read-across
Alexander-White et al.
Delineates the strict 10-step Cosmetics Europe operational criteria for blending structural analog data with in vitro assay records, establishing non-animal validation templates for raw ingredients.
Human vs. cells vs. machine: A comparative analysis of toxicological points of departure derived from quantitative read-across (qRAx), in vitro data, and in silico predictions
#research #read-across #qsar
Chiu et al.
Presents a mathematical comparison of calculated points of departure derived across advanced chemical categories, benchmarking the consistency of computerized structural stacking against active human cell responses.
Quantifying the effect of human interindividual kinetic differences on the relative potency value for riddelliine N-oxide at low dose levels by a new approach methodology
#research #toxicokinetics #pbpk
Widjaja-van den Ende et al.
Applies physiologically based kinetic simulations to scale cellular dose-responses across virtual human populations, tracking internal clearance variations to protect sensitive subgroups.
Stem Cell Reports #
An open-access journal dedicated to stem cell research, publishing significant advances in human induced pluripotent stem cell (iPSC) lines and organoid development used for patient-specific drug screening. This is an excellent site which provides advanced mechanisms to acquire information. [Link]
3D Vessels-on-Chip using isogenic hiPSC-derived VSMCs reveal NOTCH3-driven alterations in brain small vessel disease
#research #organ-on-a-chip #vascular
Vila Cuenca et al.
Deploys human induced pluripotent stem cell-derived vascular smooth muscle cells inside a microfluidic board to map out the exact cell structural alterations driving CADASIL and cerebral small vessel pathology without animal models.
A latent activated olfactory stem cell state revealed by single-cell transcriptomic and epigenomic profiling
#research #genomics #neurogenesis
Van den Berge et al.
Combines single-cell RNA sequencing with chromatin accessibility assays to resolve a hidden, pre-activated neural progenitor state that primes rapid tissue regeneration.
A simplified co-culture reveals altered cardiotoxic responses to doxorubicin in hPSC-derived cardiomyocytes in the presence of endothelial cells
#research #safety-testing #cardiology
Brescia et al.
Establishes a human cell-based microvascular-myocardial interface to demonstrate how adjacent endothelial cells modulate chemotherapeutic toxicity mechanics, fixing the false-negative blind spots of isolated single-line screens.
Advances and challenges in modeling Charcot-Marie-Tooth type 2A using iPSC-derived models
#reviews #neuroscience #pathology
Rizzuti et al.
Evaluates current human cellular platform capabilities for modeling mitochondrial optical neuropathies, identifying key operational hurdles in achieving full functional maturation in automated assay formats.
Adult hippocampal neurogenesis: New avenues for treatment of brain disorders
#reviews #neuroscience #therapeutics
Chen et al.
Reviews endogenous neural progenitor modulation pathways within the adult human dentate gyrus, outlining strategic entry points for targeted small-molecule therapeutics to reverse neurodegenerative decline.
An efficient, non-viral arrayed CRISPR screening platform for iPSC-derived myeloid and microglia models
#research #methodology #genetics
Meier et al.
Validates a high-throughput, non-viral electroporation pipeline to execute clean, arrayed gene knockouts across human macrophage and brain immune lineages simultaneously.
An in vitro model of acute horizontal basal cell activation reveals gene regulatory networks underlying the nascent activation phase
#research #genomics #regeneration
Barrios-Camacho et al.
Utilizes an advanced human airway epithelial platform to sequence the immediate transcriptional loops triggering resting progenitor cells to enter active tissue repair modes.
APOL1 risk variants induce metabolic reprogramming of podocytes in patient-derived kidney organoids
#research #organ-on-a-chip #nephrology
Song et al.
Deploys patient-specific 3D renal structures to resolve the precise metabolic shifts and podocyte structural breakdown triggered by chronic kidney disease genetic markers.
Benchmarking and optimizing Perturb-seq in differentiating human pluripotent stem cells
#research #methodology #genomics
Sivakumar et al.
Optimizes pooled CRISPR screening with single-cell transcriptomic readouts in human stem cell lineages, maximizing data capture across large functional genetic evaluations.
BMP, MEK, and WNT inhibition with NGN2 expression for rapid generation of hiPSC-derived neurons amenable to regional patterning
#research #methodology #neuroscience
Habich et al.
Unifies temporary small-molecule signaling blocks with targeted transcription factor activation to rapidly manufacture functional human neural networks with predictable spatial configurations.
Charting the translational pathway: ISSCR best practices for the development of PSC-derived therapies
#protocols #policy #clinical-translation
Barry et al.
Lays down the definitive international consensus guidelines for standardizing manufacturing, product characterization, and preclinical safety checks for human pluripotent stem cell-derived therapeutics.
Chemically defined and dynamic click hydrogels support hair cell differentiation in human inner ear organoids
#research #biomaterials #sensory
Arkenberg et al.
Validates a completely synthetic, bio-orthogonal click-chemistry matrix framework that accurately controls mechanical stiffness to drive human inner ear line maturation without animal-derived substrates.
Chromosome X dosage modulates development of aneuploidy in genetically diverse mouse embryonic stem cells
#research #genetics #stability
Stanton et al.
Examines the mechanical relationships between chromosome X copy variations and genomic structural instability across divergent mouse stem cell backgrounds.
Clonal lineage tracing and transcriptomics of cortical progenitor populations reveal maintenance of differentiation potential
#research #genomics #neurogenesis
Harkins et al.
Couples dynamic cellular barcoding with single-cell transcript mapping to prove that early cerebral progenitor cells maintain predictable, long-term multi-lineage output choices.
Computational blueprints for cell fate programming
#reviews #automation #systems-biology
Pengyi Yang
Reviews machine learning layouts and algorithmic models that scan massive multi-omic profiles to accurately calculate and predict directed cell fate conversions.
Computationally resolved neuroprogenitor cell biomarkers associate with human disorders
#research #automation #neuroscience
Cappuccio et al.
Applies predictive cluster formulas to multi-omic datasets, identifying unique surface protein markers on human neural stems that correlate directly with downstream neurodevelopmental pathology.
Connecting cilium, stress response, and proteostasis abnormalities inform variant and therapy assessment in RPGRIP1 retinal organoids
#research #diagnostics #ophthalmology
To Ha Loi et al.
Leverages human stem cell-derived 3D retinal layers to connect protein clearance faults with primary ciliary collapse, establishing an animal-free screen for blind-inducing genetic mutations.
Cranial placode differentiation defect in individuals born without a nose
#research #pathology #development
Venkoba Rao et al.
Utilizes patient-derived induced pluripotent cells to reconstruct early facial ectoderm blocks, isolating the signaling failure that arrests nasal structure creation.
Cross-modal integration of metabolomics and cardiac functionality captures dynamic metabotoxic effects of doxorubicin in engineered heart tissues
#research #safety-testing #metabolomics
Conte et al.
Unifies non-destructive micro-sensor pacing arrays with mass spectrometry to capture immediate chemical changes inside mature human cardiac microtissues during toxic oncology drug exposure.
Crosstalk via ICAM-1 enhances supportive phenotype of stellate cells and drives hepatocyte proliferation in iPSC-derived hepatic organoids
#research #organ-on-a-chip #hepatology
Mochida et al.
Demonstrates that exact cell-to-cell membrane bridges within multi-lineage liver tissue blocks control human liver cell multiplication, optimizing the structural accuracy of liver-on-a-chip boards.
Deciphering the heterogeneity of differentiating hPSC-derived corneal limbal stem cells through single-cell RNA sequencing
#research #sorting #ophthalmology
Vattulainen et al.
Profiles the single-cell transcript variations of human ocular surface systems to isolate the exact surface marker signatures needed for high-purity selection of therapeutic eye-repair stem layers.
Derivation and analysis of human somatic sensory neuron subtypes facilitated through fluorescent hPSC reporters
#research #methodology #neuroscience
Malka-Gibor et al.
Engineers dual-fluorescent human reporter cell matrices to isolate and study discrete sensory cell groups, building high-resolution platforms for human-relevant chronic pain screening.
Derivation of transplantable human thyroid follicular epithelial cells from induced pluripotent stem cells
#research #tissue-engineering #endocrinology
Undeutsch et al.
Achieves efficient manufacturing of pure, functional human thyroid units capable of organizing into vascularized structure loops, offering an advanced option to study hormone regulation in vitro.
Dietary restriction mitigates 5-fluorouracil-induced thrombocytopenia in aged mice via mitochondrial potentiation in hematopoietic stem cells and megakaryocyte progenitors
#research #metabolism #hematopoiesis
Qiu et al.
Tracks metabolic pathways inside aging blood stem systems, proving that nutrient stress loops alter mitochondrial energy output to protect bone marrow cells from cytostatic chemotherapy damage.
Dissecting cardiovascular disease-associated noncoding genetic variants using human iPSC models
#reviews #cardiology #genomics
Dababneh et al.
Reviews progress in human cell models used to decode complex mutations located in non-protein-coding regions, isolating the target loops driving structural heart failures.
Dissecting microglial contributions to neurodegenerative disease pathophysiology using human pluripotent stem cells
#reviews #neuroscience #immunology
Dayoung Kim et al.
Examines the technical integration of patient-derived immune lines into multi-lineage central nervous system models, tracing inflammatory pathways in Alzheimer’s and Parkinson’s profiles without animals.
Diversity of cortical progenitors directs neuronal layer formation and regional glial patterning
#research #neurogenesis #development
Ojalvo-Sanz et al.
Traces early cell origin fields inside cerebral constructs, showing that pre-determined progenitor groups independently layout the stratified human brain architecture.
Diversity-in-a-dish: A practical framework for hiPSC model development
#reviews #safety-testing #methodology
Weidema et al.
Outlines a comprehensive strategic plan to construct patient cell validation biobanks that reflect varied human genetics, aiming to eliminate population data blind spots in preclinical drug evaluations.
Dynamic governance: A new era for consent for stem cell research
#policy #ethics #data-autonomy
Isasi et al.
Proposes a responsive, technology-driven data management setup that gives patients continuous oversight and approval choices for their digital multi-omic records and biomaterials.
Efficient differentiation of human iPSCs into Leydig-like cells capable of long-term stable secretion of testosterone
#research #bioprocessing #endocrinology
Sato et al.
Details a robust manufacturing protocol to differentiate human stem cell collections into endocrine cells that sustain steady androgen production, establishing human alternatives for male reproductive safety testing.
Elevated hematopoietic stem cell frequency in mouse alveolar bone marrow
#research #official-repository #hematopoiesis
Niizuma et al.
Identifies jawbone tissue segments as a unique, cell-rich bone marrow location that contains high concentrations of blood stem cell groups with distinct protective niches.
Ependymal and neural stem cells are close relatives
#reviews #neuroscience #cell-biology
Lokka et al.
Traces shared structural genes and signal lineages connecting ventricular wall line systems with core neuroprogenitor blocks, mapping out cellular targets for brain tissue repair.
Exit from naive pluripotency proceeds with variable latency but without asymmetric division to generate population heterogeneity
#research #cell-biology #biophysics
Strawbridge et al.
Applies deep mathematical modeling and continuous tracking to trace stem cells as they shift from ground states, showing that cell profile variations emerge from time delays rather than physical cell splitting choices.
Extracellular-vesicle-mediated transfer of let-7b/7c promotes the proliferation of transition-state spermatogonia in neonatal mouse testis
#research #sensory #reproductive
Zheng et al.
Demonstrates that microscopic chemical transport carriers inside early support niches cross membrane barriers to deliver regulatory microRNA loops, triggering germ cell division blocks.
From responsibility to responsibilization in stem cell research: An ethical framework
#policy #ethics #methodology
Assen et al.
Establishes a logical ethical architecture to evaluate how regulatory duties shift between developers and system users across advanced human developmental research fields.
Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation
#research #genetics #stability
Raposo et al.
Traces token epigenomic parameters across female cell lines to chart long-term expression variance, establishing analytical baselines for quality checking in clinical cell therapy bioprocessing.
Genetics of growth rate in induced pluripotent stem cells
#research #genomics #automation
Brian Lee et al.
Scans genetic loci variations across massive cell file collections to map out the exact baseline locus regulators controlling cell growth, optimizing industrial human stem cell bioprocessing runs.
Genome-edited safe and immune-evasive human pluripotent cells: Potential solution for allogeneic therapies
#research #biotechnology #immunology
Tam et al.
Uses CRISPR multiplexing to knock out HLA expression loops while adding protective surface proteins, creating universal, immune-shielded human master seed lines for allogeneic tissue therapies.
Genome-wide screening reveals essential roles for HOX genes and imprinted genes during caudal neurogenesis of human embryonic stem cells
#research #genomics #neurogenesis
Kinreich et al.
Deploys functional genome-wide CRISPR screens to determine the essential transcriptional networks and homeobox gene networks that direct stem lines toward spinal cord neural paths.
Graft-derived horizontal cells contribute to host-graft synapses in degenerated retinas after retinal organoid transplantation
#research #sensory #ophthalmology
Watanabe et al.
Uses high-resolution tracking inside organoid transplant models to prove that graft cells physically weave functional synaptic connections with severely broken sensory networks.
GSK3α negatively regulates GSK3β by decreasing its protein levels and enzymatic activity in mouse embryonic stem cells
#research #cell-biology #cell-signaling
Duo Wang et al.
Examines internal feedback pathways within the glycogen synthase kinase axis, clarifying the precise balancing steps that regulate stem cell self-renewal states.
Hematopoietic organoids: Opportunities and challenges in modeling human hematopoiesis and diseases in vitro
#reviews #organ-on-a-chip #hematopoiesis
Liming Du et al.
Reviews advanced 3D structural strategies used to recreate functional bone marrow niches, detailing a clear non-animal track to model complex blood cancers and safe screening loops.
How neural stem cell therapy promotes brain repair after stroke
#reviews #neuroscience #therapeutics
Weber et al.
Examines the functional healing steps used by engineered neural cell sheets to clear debris and reconstruct neural circuits following ischemic brain injury events.
Human cone photoreceptor transplantation stimulates remodeling and restores function in AIPL1 model of end-stage Leber congenital amaurosis
#research #sensory #ophthalmology
Procyk et al.
Transplants high-purity human stem cell-derived vision receptors into end-stage genetic loss targets, proving functional connection and repair of blind sensory matrices.
Human macula formation involves two waves of retinoic acid suppression via CYP26A1 that modulate cell cycle exit and cone subtype specification
#research #sensory #development
Harding et al.
Deploys human retinal organoids to trace the precise chemical timeline of morphogen blockades that establish the high-acuity human center vision spot, highlighting development tracks absent in small rodent formats.
Human organoids: Fit for drug discovery?
#reviews #safety-testing #organ-on-a-chip
Wittich et al.
Presents a strict logical evaluation of 3D human organoid platforms across large screening runs, defining standard readiness metrics to replace legacy toxicology models in corporate pipelines.
Hypersynchronous iPSC-derived SHANK2 neuronal networks are rescued by mGluR5 agonism
#research #neuroscience #pharmacology
McCready et al.
Deploys high-density multi-electrode arrays with autism-linked patient cell lines to uncover network timing faults, validating a chemical patch route to restore balance without animal testing.
Identifying enabling strategies for effective public dialogue in human embryo research
#policy #ethics #engagement
Beckett et al.
Outlines practical methods to run transparent open discussions and framework checks regarding recent progress in human developmental and synthetic embryo research.
Improving rigor and reproducibility through implementation of the ISSCR standards for human stem cell use in research
#protocols #policy #quality-control
Fischer et al.
Establishes clear laboratory reporting criteria and characterization checklists based on recent ISSCR global agreements, standardizing cell file checks to maximize reproducibility.
Impulse initiation in engrafted pluripotent stem cell-derived cardiomyocytes can stimulate the recipient heart
#research #cardiology #therapeutics
Stüdemann et al.
Details the pacing mechanics and electrical coupling patterns of transplanted human cardiac patch units, proving direct functional drive over host muscle blocks.
In situ spatial transcriptomics reveals novel markers of the limbal stem cell niche and ocular surface epithelia
#research #genomics #ophthalmology
Nureen et al.
Deploys high-resolution spatial transcript mapping directly on clean tissue slices to locate hidden protective genes that shield and preserve eye surface stem populations.
Inhibition of N-myristoyltransferase in pluripotent stem cells promotes the naive state in mice and elicits trophectoderm and primitive endoderm markers in humans
#research #cell-biology #biotechnology
Yoshida et al.
Discovers that blocking protein fatty-acid attachments modifies core cell states, triggering unique structural choices that enable the creation of clean extra-embryonic lineages.
In and out: Benchmarking in vitro, in vivo, ex vivo, and xenografting approaches for an integrative brain disease modeling pipeline
#reviews #methodology #neuroscience
Pereira et al.
Presents a critical comparative review of central nervous system research strategies, highlighting how human cell arrays and microphysiological systems accurately resolve the biological blind spots of traditional animal configurations.
JAK/STAT signaling promotes the emergence of unique cell states in ulcerative colitis
#research #organ-on-a-chip #gastroenterology
Maciag et al.
Uses patient-derived gut organoid loops to trace out the inflammatory signaling vectors that reshape internal tissue lining cells during active colitis disease states.
MECP2 mutations rewire human ESC fate and bias cortical lineage commitment
#research #neuroscience #genomics
Guillon et al.
Examines how single-point epigenetic gene modifications alter early stem cells, showing a bias that disrupts layered cortical formation and replicates Rett syndrome traits.
METTL3 uncouples chromatin accessibility from transcription during retinal development
#research #genomics #sensory
Jing Xu et al.
Discovers that RNA methylation enzymes act as independent switches separating structural DNA exposure states from active transcription during human retinal specialized cell formation.
Molecular signature of human endometrial stem/progenitor cells at the single-cell level
#research #genomics #gynaecology
Fitzgerald et al.
Resolves the single-cell gene tracking patterns of internal tissue lining networks, identifying high-purity somatic markers to build reliable human model assays for reproductive health research.
Modeling common Alzheimer’s disease with high and low polygenic risk in human iPSC: A large-scale research resource
#research #genomics #neuroscience
Maguire et al.
Assembles a major open-source human cell biobank grouping lines by computed polygenic risk scores, delivering an animal-free framework to screen sporadic Alzheimer’s variations.
Modeling diabetic alpha cell dysfunction using stem cell-derived alpha cells
#research #organ-on-a-chip #endocrinology
Shrestha et al.
Differentiates human stem cell lines into functional glucagon-secreting blocks to study metabolic failures under diabetic chemical loads, avoiding traditional animal injection series.
Neural stem cells of the subventricular zone: A potential stem cell pool for brain repair in Parkinson’s disease
#reviews #neuroscience #therapeutics
Verkerke et al.
Maps out the localized signal networks within brain lining niches that could be chemically triggered to stimulate internal cellular repair loops in Parkinson’s pathology.
Nuclear Lipin1 recruits HDAC2 to epigenetically repress SREBP1-dependent lipid synthesis and myelination in hypoxic preterm white matter injury
#research #neuroscience #pathology
Xinyu Li et al.
Resolves the precise biochemical docking track where fat-processing enzymes join structural adaptors to turn off brain insulation construction under early oxygen-deprived conditions.
“Oxygen tone” drives stage-specific OPC phenotypes for cell-based stroke therapy
#reviews #neuroscience #therapeutics
Kokaia & Palma-Tortosa
Analyzes how precise oxygen concentration gradients inside cell processors direct neural precursor properties, optimization rule tracks for therapeutic production.
PKM2 is a key factor to regulate neurogenesis and cognition by controlling lactate homeostasis
#research #metabolism #neuroscience
Pengyan He et al.
Identifies metabolic enzyme adaptors that regulate local lactic balance, demonstrating a molecular mechanism that controls new nerve creation and memory preservation.
Post-replicative chromatin accessibility predicts cell fate change
#research #cell-biology #genomics
Knudsen et al.
Implements single-cell sequencing tracks to prove that structural DNA openness immediately following division states dictates downstream multi-lineage differentiation possibilities.
Pro-repair properties of a human embryonic stem cell-derived astrocyte cell therapy in demyelinating disorders
#research #neuroscience #therapeutics
Sofer Stepanov et al.
Validates an advanced human astrocyte cellular line that travels through broken tissue patches to clear inflammatory debris and accelerate myelin sheet reconstruction.
Probing DNA damage in Rett syndrome neurons uncovers a role for MECP2 regulation of PARP1
#research #neuroscience #genomics
Morales et al.
Deploys human stem cell neuro-arrays to show that a lack of methyl-binding proteins leaves human neurons vulnerable to double-strand breaks by failing to regulate primary repair machines.
Progenitor neighborhoods function as transient niches to sustain olfactory neurogenesis
#research #neurogenesis #cell-biology
Rajan et al.
Documents that localized spatial grouping patterns among adjacent early cells function as a short-term supportive home zone to maintain ongoing sensory cell replacement.
Promoting the adoption of best practices and standards to enhance quality and reproducibility of stem cell research
#policy #protocols #quality-control
Selfa Aspiroz et al.
Outlines rigorous consensus criteria for establishing international human cell validation standards, aiming to enhance multi-site data consistency for global NAM acceptance.
Proteomics-based receptor-ligand matching enhances differentiation maturity of human-stem-cell-derived neurons
#research #methodology #proteomics
Dimitrov et al.
Applies massive target protein scans to map active surface ports on developing human neurons, matching them with defined chemical mixtures to accelerate system functional maturation.
Rat cell-derived kidney generation via interspecies blastocyst complementation in an Osr1-KO mouse model
#research #genetics #development
Yuri & Isotani
Uses targeted transcription gene knockouts to create a structural template gap in early developmental blocks, testing how diverse cell inputs interact across different species lines.
Reconstitution of the cellular niche requirements for primordial germ cell-like cell progression in humans
#research #cell-biology #reproductive
Chang et al.
Constructs an animal-free multi-lineage cell network that recreates the precise structural signals required to support early human reproductive cell line progression in vitro.
Response to Yu et al.
#letters #methodology
Yuanyue Liu et al.
Technical clarification note addressing validation standards and data interpretation limits across human cellular reprogramming setups.
Rod-shaped micropatterning enhances the electrophysiological maturation of cardiomyocytes derived from human induced pluripotent stem cells
#research #methodology #cardiology
Al Sayed et al.
Employs high-precision microscale geometric stamping to force human heart cells into structural alignment, driving functional sarcomere assembly and predictable pacing actions without animal tissue.
Skeletal muscle-on-a-chip in microgravity as a platform for regeneration modeling and drug screening
#research #organ-on-a-chip #aerospace
Soochi Kim et al.
Launches a specialized fluid micro-chamber board onto space stations to study microgravity-induced muscle wasting, establishing an advanced human testing format for accelerated aging research.
STARD10 regulates human pancreatic β cell differentiation and triglyceride metabolism
#research #metabolism #endocrinology
Tan et al.
Isolates the specific lipid-transfer proteins that handle fat balance inside human insulin-secreting cells, offering a defined mechanism track linked to metabolic strain events.
Suppression of ATM kinase signaling accelerates cellular senescence
#research #cell-biology #aging
Ishikawa et al.
Blocks key nuclear warning systems in human neuroprogenitor arrays to replicate rapid gene-driven aging traits, mapping pathogenetic tracks without live rodent cross-aging chains.
The emergence of electrical activity in human brain organoids
#reviews #neuroscience #organ-on-a-chip
Mancinelli et al.
Reviews recent breakthroughs in recording synchronized network pacing inside 3D human neural layers, evaluating hardware setups for non-destructive long-term signal tracking.
The ethical aspects of human organ-on-chip models: A mapping review
#reviews #ethics #policy
Weidema et al.
Presents a systematic map of international ethical and regulatory questions surrounding advanced microphysiological systems, linking donor tracking choices directly with future industrial standard plans.
The regenerative role of neural crest stem cells in physical stimuli-enhanced peripheral nerve repair
#research #engineering #neuroscience
Tai et al.
Deploys specialized electric-field plates over human nerve stem matrices, proving that physical force fields double axonal extension rates and specify neuroglial choices.
The roles of TGF-β, Wnt, and MAPK signaling pathways in joint lineage specification in vitro and ex vivo
#research #cell-signaling #orthopedics
Suyash Raj et al.
Maps the interconnected pathway circuits that govern human limb stem cells as they select cartilage or joint line fates, establishing a reliable baseline recipe for orthopedic NAM boards.
Three-dimensional co-culturing reveals human stem cell-derived somatostatin interneurons with subclass expression
#research #organ-on-a-chip #neuroscience
Bruzelius et al.
Integrates distinct inhibitory cell lines into 3D human brain structures to manufacture complex neural networks, providing human-relevant assay models for epilepsy drug screening.
Toward standardized iPSC testing: Insights from a multi-year international Quality Assessment Round
#research #policy #quality-control
Hägg et al.
Analyzes data from a major cross-border testing trial across global labs, building uniform baseline metrics for genetic check tracking to guarantee safe allogeneic cell line entry.
Transplantation of genome-edited retinal organoids restores some fundamental physiological functions coordinated with severely degenerated host retinas
#research #sensory #ophthalmology
Watanabe et al.
Transplants CRISPR-corrected 3D human photoreceptor layers into severely damaged eye fields, proving that the structured human layers successfully parse light signals and connect with host sensory channels.
Validation of non-destructive morphology-based selection of cerebral cortical organoids by paired morphological and single-cell RNA-seq analyses
#research #automation #quality-control
Ikeda et al.
Validates a smart computer-vision pipeline that scans raw 3D organoid shapes to identify high-quality neural structure lines without needing destructive gene-tag checks.
XPRESSO: Rapid genetic engineering of human pluripotent stem cells for durable overexpression using a modular anti-silencing vector
#research #biotechnology #methodology
Wexler et al.
Introduces a modular plasmid toolkit (XPRESSO) designed with specialized boundary elements that block chromatin locking, ensuring stable and long-term gene expression in human cell arrays.
Toxicology in Vitro #
A leading peer-reviewed journal focused on the application of in vitro and in silico systems to assess the toxicity of chemical substances and understand cellular mechanisms of action without animal models. [Link]
Assessing the skin sensitization potential of fragrance ingredients in consumer products using the peroxidase peptide reactivity assay (PPRA) as an additional weight of evidence
#research #safety-testing #dermatology
Schember et al.
Validates the execution parameters of the non-animal Peroxidase Peptide Reactivity Assay (PPRA) within a larger weight-of-evidence safety tier, ensuring highly accurate skin allergy profiling for complex lipophilic fragrance compounds.
Integrate mechanistic evidence from new approach methodologies (NAMs) into a read-across assessment to characterise trends in shared mode of action
#research #safety-testing #read-across
Escher et al.
Establishes a structured data-integration workflow within read-across frameworks, demonstrating how mechanistic in vitro profiles verify chemical group similarities and define mode-of-action boundaries to minimize regulatory uncertainty.
NAM-based development of a predictive test model for evaluating skin mildness potential of rinse-off products via integrated in vitro assays
#research #safety-testing #dermatology
Ng et al.
Unifies a multi-assay animal-free testing tier to accurately map consumer product skin mildness kinetics, replacing legacy cosmetic patch-testing with integrated cellular markers.
Qualification of a non-animal vaginal irritation method admitted as nonclinical assessment model (NAM) in the Incubator Phase of the United States Food and Drug Administration (US FDA) Medical Devices Development Tool (MDDT)
#research #safety-testing #policy
Costin et al.
Documents the formal qualification milestones of a fully human reconstructed epithelial model within the US FDA MDDT track, establishing a benchmark pathway for replacing mammalian irritation assays in regulatory device registration.
Various #
These are items of interest from different journals. A sufficient number of articles were not found to warrant a specific journal’s heading yet, so all these articles are placed under the Various heading. Over time, we will investigate such journals further and if the quantity of publications are sufficient, they will be re-organized appropriately.
A case study with benzoic acid demonstrating how integration of In Silico Tools, mechanistic In Vitro NAM, and toxicological data improves definition of point of departure for a chemically defined class in read-across assessments
#research #safety-testing #read-across
Lester et al.
Deploys benzoic acid as a structural template to demonstrate how combining computational modeling with targeted cell assays defines strict molecular points of departure for structural chemical classes.
AI-driven parametrization of Michaelis–Menten maximal velocity: Advancing in silico new approach methodologies (NAMs)
#research #automation #toxicokinetics
Karakoltzidis et al.
Applies machine learning algorithms to calculate maximal enzyme velocity values ($V_{max}$), automating metabolic clearance predictions for high-throughput chemical risk evaluations.
Determination of acceptable margins of exposure (MoE) for NAM-based skin sensitisation risk assessment
#research #safety-testing #policy
Joe Reynolds et al.
Defines the boundary criteria and safety factors needed to calculate secure human exposure limits using non-animal test datasets.
Non-clinical human neural new approach methodologies (NAMs): Electrophysiological assessment of opioid agonist and antagonist combination
#research #safety-testing #neuroscience
Serna et al.
Deploys micro-electrode boards with human stem-derived nerve networks to monitor functional electrical patterns, capturing precise pharmacology details without rodent neural assays.
Non-animal new approach methodologies (NAMs): Increasingly effective in validated contexts, more ethical and more economically productive
#reviews #policy #economics
Elliott Johnson et al.
Presents a multi-layered logical evaluation of alternative data models, detailing how animal-free approaches lower pipeline run costs while producing human-relevant toxicological outcomes.
Pursuing societal impact in research projects on NAM development: An analysis of routes to impact
#reviews #policy #translation
Wijne et al.
Tracks the structural paths that take new non-animal models from basic laboratory design to operational adoption by global chemical companies.
Sensitivity analysis of the inputs for bioactivity-exposure ratio calculations in a NAM-based systemic safety toolbox
#research #toxicokinetics #uncertainty
Lin et al.
Applies variance equations to parameters used in cell-to-body data conversions, tracking down the exact variable blocks that introduce numerical errors in safe dose calculations.
TOXTRUST: a tool leveraging the Dempster-Shafer Theory for robust integration of NAM results in decision-making considering uncertainty
#research #automation #uncertainty
Kopańska et al.
Engineers a predictive software utility (TOXTRUST) that applies belief-function mathematical modeling to combine conflicting results from alternative assays, projecting a clear security baseline.
Unlocking the future of environmental safety: a framework for integrating new approach methodologies in decision-making
#reviews #policy #ecotoxicology
Rivetti et al.
Proposes a clear operational pathway for regulatory authorities to blend non-animal data streams into ecological protection metrics, speeding up compound safety categorizations.